Characterization of a small mobilizable transposon, MTnSag1, in Streptococcus agalactiae.

نویسندگان

  • Adeline Achard
  • Roland Leclercq
چکیده

The transposon MTnSag1 from Streptococcus agalactiae carried an IS1-like transposase gene and the lnu(C) gene, which encoded a lincosamide nucleotidyltransferase. MTnSag1 could be mobilized by the conjugative transposon Tn916. An intermediate circular form of MTnSag1 and a putative origin of transfer at the 3' end of the lnu(C) gene were characterized.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Conjugative transfer and cis-mobilization of a genomic island by an integrative and conjugative element of Streptococcus agalactiae.

Putative integrative and conjugative elements (ICEs), i.e., genomic islands which could excise, self-transfer by conjugation, and integrate into the chromosome of the bacterial host strain, were previously identified by in silico analysis in the sequenced genomes of Streptococcus agalactiae (M. Brochet et al., J. Bacteriol. 190:6913-6917, 2008). We investigated here the mobility of the elements...

متن کامل

Prevalence of Virulence Factors and Antimicrobial Resistance of Streptococcus agalactiae and Streptococcus uberis in Ruminant Sub-clinical Mastitic Milk in Iran

Background and Aims: The aim of this study was to determine the virulence and antibiotic resistance properties of Streptococcus agalactiae (S. agalactiae) and Streptococcus uberis (S. uberis) isolated from the ruminant’s sub-clinical mastitic milk in Iran. Materials and Methods: Positive samples in the CMT test were cultured and S. agalactiae and S. uberis have been identified using bi...

متن کامل

Streptococcal group B integrative and mobilizable element IMESag-rpsI encodes a functional relaxase involved in its transfer

Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacteri...

متن کامل

Biofilm formation, hemolysin production and antimicrobial susceptibilities of Streptococcus agalactiae isolated from the mastitis milk of dairy cows in Shahrekord district, Iran

Streptococcus agalactiae is a major contagious pathogen causing bovine sub-clinical mastitis. The present investigation was carried out to determine some phenotypic characteristics of the S. agalactiae strains isolated from bovine mastitis cases in dairy cows of Shahrekord in the west-center of Iran. One hundred eighty California mastitis test (CMT) positive milk samples were ...

متن کامل

The Rapid CAMP Test for Identification of Streptococcus agalactiae Using Alpha Toxin

Alpha toxin derived from Clostridium perfringens was used for diagnosis of Streptococcus agalactiae (St.agalactiae) by rapid and spot CAMP tests. The selected Cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by DEAE cellulose chromatography. The efficacy of both purified and crude α-toxins were examined by rapid...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of bacteriology

دوره 189 11  شماره 

صفحات  -

تاریخ انتشار 2007