1 Kinesin - 73 Is a Processive Motor That Localizes to Rab 5 - Containing Organelles
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KINESIN-73 IS A PROCESSIVE MOTOR THAT LOCALIZES TO RAB5-CONTAINING ORGANELLES Thomas M. Huckaba, Arne Gennerich, James E. Wilhelm, Athar H. Chishti, and Ronald D. Vale Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158-2517, Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, Department of Physiology, Tufts University School of Medicine, Boston MA 02111, and Howard Hughes Medical Institute Running Title: Characterization of Kinesin-73 Address correspondence to: Ronald D. Vale, PhD, 600 16 Street, San Francisco, CA 94158-2517. Fax: 415-476-5233; E-mail: [email protected]
منابع مشابه
Kinesin-73 Is a Processive Motor That Localizes to Rab5-containing Organelles*
Drosophila Kinesin-73 (Khc-73), which plays a role in mitotic spindle polarity in neuroblasts, is a metazoan-specific member of the Kinesin-3 family of motors, which includes mammalian KIF1A and Caenorhabditis elegans Unc-104. The mechanism of Kinesin-3 motors has been controversial because some studies have reported that they transport cargo as monomers whereas other studies have suggested a d...
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Kinesin-3 motors drive the transport of synaptic vesicles and other membrane-bound organelles in neuronal cells. In the absence of cargo, kinesin motors are kept inactive to prevent motility and ATP hydrolysis. Current models state that the Kinesin-3 motor KIF1A is monomeric in the inactive state and that activation results from concentration-driven dimerization on the cargo membrane. To test t...
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Mammalian KIF3AC contains two distinct motor polypeptides and is best known for its role in organelle transport in neurons. Our recent studies showed that KIF3AC is as processive as conventional kinesin-1, suggesting that their ATPase mechanochemistry may be similar. However, the presence of two different motor polypeptides in KIF3AC implies that there must be a cellular advantage for the KIF3A...
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Kinesins perform mechanical work to power a variety of cellular functions, from mitosis to organelle transport. Distinct functions shape distinct enzymologies, and this is illustrated by comparing kinesin-1, a highly processive transport motor that can work alone, to Eg5, a minimally processive mitotic motor that works in large ensembles. Although crystallographic models for both motors reveal ...
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تاریخ انتشار 2010