Studies on resistance against 5-fluorouracil. II. Thymidylate synthetase from drug-resistant tumor lines.
نویسنده
چکیده
A method is described for the assay of thymidylate synthetase activity in crude ex tracts of Ehrlich ascites tumor. The method involves the use of tritiated deoxyuridine5'-phosphate as substrate. The inhibition of thymidylate synthetase by 5-fluorodeoxyuridine-5'-phosphate was measured in extracts from one 5-fluorouracil-sensitive and four drug-resistant lines of Ehrlich ascites tumor. A high degree of inhibition was observed in all cases, and no difference between the drug-sensitive and -resistant lines was apparent.
منابع مشابه
Studies on Resistance against 5-Fluorouracil* II. Thymidylate Synthetase from Drug-resistant Tumor Lines
A method is described for the assay of thymidylate synthetase activity in crude ex tracts of Ehrlich ascites tumor. The method involves the use of tritiated deoxyuridine5'-phosphate as substrate. The inhibition of thymidylate synthetase by 5-fluorodeoxyuridine-5'-phosphate was measured in extracts from one 5-fluorouracil-sensitive and four drug-resistant lines of Ehrlich ascites tumor. A high d...
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عنوان ژورنال:
- Cancer research
دوره 22 شماره
صفحات -
تاریخ انتشار 1962