Glucokinase of Rabbit Liver PURIFICATION AND PROPERTIES* Josti SALAS,~ MARGARITA SALAS,$ ELADIO VIRUELA,J AND ALBERTO SOLS
It has been recently shown that in rat liver there are two enzymes which phosphorylate glucose to glucose 6-phosphate. One of them is a hexokinase with a great affinity for glucose and, like those of other animal tissues it is inhibited by glucose 6phosphate; the other one, named glucokinase, has a low affinity for glucose, is not inhibited by glucose 6phosphate, and appears to be responsible for glycogen synthesis from glucose in liver (1). Hexokinase activity exists in both fetal and adult liver whereas glucokinase develops after birth (2). Results from several laboratories have shown that glucokinase activity disappears as a result of fasting and diabet,es and reappears after refeeding or insulin administration, respectively (l-9), and that this reappearance involves synthesis de nouo of the enzyme apparently mediated by insulin (3, 4, 6, 10). This paper describes the purification of rabbit liver glucokinase about 200-fold and some properties of the enzyme. Rabbit liver glucokinase is activated by sulfhydryl reagents and is protected by potassium ions. Besides glucose, mannose and 2-deoxyglucose are good substrates of the glucokinase. Glucosamine and several N-substituted derivatives are strong competitive inhibitors of the glucokinase. Adenosine diphosphate inhibits the enzyme; this inhibition is only partially reversed by adenosine triphosphate.
Rational Design of a Carrier Protein for the Production of Recombinant Toxic Peptides in Escherichia coli
Commercial uses of bioactive peptides require low cost, effective methods for their production. We developed a new carrier protein for high yield production of recombinant peptides in Escherichia coli very well suited for the production of toxic peptides like antimicrobial peptides. GKY20, a short antimicrobial peptide derived from the C-terminus of human thrombin, was fused to the C-terminus o...متن کامل
Full: HTML / PDF (935K) Select Citation A New Procedure To Measure the Antioxidant Activity of Insoluble Food Components Arda Serpen, Edoardo Capuano, Vincenzo Fogliano, and Vural Gökmen pp 7676 7681; (Article) DOI: 10.1021/jf071291z Abstract Full: HTML / PDF (726K) Select Citation http://pubs3.acs.org/acs/journals/toc.page?incoden=jafcau&indecade=0&involume=55&inissue=19 (6 of 19)24/4/2551 11:...متن کامل
Despite the fact that Leishmania parasites are transmitted exclusively by the bite of an infected sand fly, there are few reports concerning the relationship of this factor on Leishmania infection. Two models were developed, one using needle injection and the second based on natural transmission by the vector. During the natural transmission of Leishmania, sand flies inoculate low numbers of me...متن کامل
By incorporating both majorization theory and stochastic dominance theory, this paper presents a general theory and a unifying framework for determining the diversification preferences of risk-averse investors and conditions under which they would unanimously judge a particular asset to be superior. In particular, we develop a theory for comparing the preferences of different convex combination...متن کامل
BACKGROUND Adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. Despite immunostimulatory properties, the use of bacterial lipopolysaccharide (LPS) as an adjuvant has been hampered due to its toxicity and pyrogenicity. Brucella abortus LPS is less toxic and has no pyrogenic properties compared to LPS from other gram negati...متن کامل