Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography.
نویسندگان
چکیده
VOL. 79 0091 hts reserved. 125 DOI: 10.1016/S0091 -679X -679X II. R ationale III. M ethods A. H igh-Pressure Freezing, Freeze-Substitution, and Embedding B. P reparation of Sections for EM C. I mage Acquisition and Tomographic Reconstruction D. 3 D Modeling of the Basal Body Complex IV. I nstrumentation and Materials A. H igh-Pressure Freezing and Freeze-Substitution B. E lectron Tomography C. T omographic Reconstruction and Modeling V. D iscussion VI. S ummary R eferences
منابع مشابه
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عنوان ژورنال:
- Methods in cell biology
دوره 79 شماره
صفحات -
تاریخ انتشار 2007