An improved method for the purification of staphylococcal nuclease.
نویسندگان
چکیده
Commercial staphylococcal nuclease (Foggi strain) containing small amounts of contaminating protein can be purified by chromatography on phosphorylated cellulose columns. The purified enzyme is free of acid and alkaline phosphatase activities. It is homogeneous by polyacrylamide gel electroptfresis, and antigenically pure by immunodiffusion. The E” 1 Cm of the pure enzyme at neutral pH is 9.3 at 280 rnp and 9.7 at 277 mp.
منابع مشابه
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 242 20 شماره
صفحات -
تاریخ انتشار 1967