Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing.
نویسندگان
چکیده
INTRODUCTION We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009. METHODOLOGY This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. RESULTS From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing. CONCLUSIONS The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.
منابع مشابه
Highly sensitive and quantitative detection of the H274Y oseltamivir resistance mutation in seasonal A/H1N1 influenza virus.
A C-to-T transition mutation in the neuraminidase gene from seasonal A/H1N1 causes a His-to-Tyr mutation at amino acid position 275 (H274Y, universal N2 numbering), conferring resistance against oseltamivir (Tamiflu). This mutation was first detected in clinical samples in Europe during the 2007-2008 influenza season. Viruses with this mutation reached a prevalence of ∼11% by the end of the sea...
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عنوان ژورنال:
- Journal of infection in developing countries
دوره 5 5 شماره
صفحات -
تاریخ انتشار 2011