Genetic and Chemical Profiling of Gymnema sylvestre Accessions from Central India: Its Implication for Quality Control and Therapeutic Potential of Plant
نویسندگان
چکیده
BACKGROUND Gymnema sylvestre, a vulnerable plant species, is mentioned in Indian Pharmacopeia as an antidiabetic drug. OBJECTIVE Study of genetic and chemical diversity and its implications in accessions of G. sylvestre. MATERIALS AND METHODS Fourteen accessions of G. sylvestre collected from Central India and assessment of their genetic and chemical diversity were carried out using ISSR (inter simple sequence repeat) and HPLC (high performance liquid chromatography) fingerprinting methods. RESULTS Among the screened 40 ISSR primers, 15 were found polymorphic and collectively produced nine unique accession-specific bands. The maximum and minimum numbers of amplicones were noted for ISSR-15 and ISSR-11, respectively. The ISSR -11 and ISSR-13 revealed 100% polymorphism. HPLC chromatograms showed that accessions possess the secondary metabolites of mid-polarity with considerable variability. Unknown peaks with retention time 2.63, 3.41, 23.83, 24.50, and 44.67 were found universal type. Comparative hierarchical clustering analysis based on foresaid fingerprints indicates that both techniques have equal potential to discriminate accessions according to percentage gymnemic acid in their leaf tissue. Second approach was noted more efficiently for separation of accessions according to their agro-climatic/collection site. CONCLUSION Highly polymorphic ISSRs could be utilized as molecular probes for further selection of high gymnemic acid yielding accessions. Observed accession specific bands may be used as a descriptor for plant accessions protection and converted into sequence tagged sites markers. Identified five universal type peaks could be helpful in identification of G. sylvestre-based various herbal preparations. SUMMARY Nine accession specific unique bandsFive marker peaks for G. sylvestre.Suitability of genetic and chemical fingerprinting Abbreviations used: HPLC: High Performance Liquid Chromatography, ISSR: Inter Simple Sequence Repeats, CTAB: Cetyl Trimethylammonium Bromide, DNTP: Deoxynucleotide Triphosphates.
منابع مشابه
Screening of Various Solvent Extracts of Gymnema sylvestre R.Br. Leaf for Antibacterial Activity
INTRODUCTION Plants have always played an important role for mankind especially as food and medicine. In the last few decades, there has been an exponential growth in the field of herbal medicine due to failure of modern medicine in providing effective treatment for chronic diseases and emergence of multidrugresistant bacteria. Various extracts from traditional medicinal plants with folklore re...
متن کاملRole of Gymnema sylvestre as Alternative Medicine
Context: Gymnema sylvestre R.Br, belonging to the family Asclepiadaceae, has been employed to control diabetes, obesity, atherosclerosis etc., by traditional medicinal practitioners of India for nearly two millennia. The active principals from the leaf extracts is assumed to be gymnemic acid and its derivatives which are of triterpenoids in chemical nature have the ability to renew the islet ce...
متن کاملTriterpenoids from Gymnema sylvestre and their pharmacological activities.
Because plants are estimated to produce over 200,000 metabolites, research into new natural substances that can be used in the pharmaceutical, agrochemical and agro-industrial production of drugs, biopesticides and food additives has grown in recent years. The global market for plant-derived drugs over the last decade has been estimated to be approximately 30.69 billion USD. A relevant specific...
متن کاملAssessment of genetic diversity and relationship of coastal salt tolerant rice accessions of Kerala (South India) using microsatellite markers
Rice (Oryza sativa L.) is one of the most significant cereal crops, about 3 billion people, nearly half the world's population; depend on rice for survival and it offers up to 80% of daily energy intake in most of the Asian countries. Knowledge of the distribution, extent and pattern of genetic variation is useful for estimation of any possible loss of genetic diversity and its role in breeding...
متن کاملAntibacterial Activity of Gymnema kollimalayanum, A New Plant from Peninsular India
Gymnema kollimalayanum A. Ramachandran and M.B. Viswan (Asclepiadaceae) is a new plant found in Kolli hills, Eastern Ghats, Peninsular India. The present investigation deals with the antibacterial potentials of the aqueous and organic solvents extracts from powdered leaves of G. kollimalayanum were tested against eight Gram-negative (Klebsiella pneumoniae, Proteus vulgaris, Salmonella paratyphi...
متن کامل