A specific anti-proinsulin serum and the presence of proinsulin in calf serum.
نویسندگان
چکیده
A specific antibody against proinsulin has been obtained by adsorbing the original anti-proinsulin guinea pig serum with a solid immunosorbent of Sephadex-insulin. The specificity of the antibody against antigenic determinants of proinsulin was established by radioimmunoassay and by passive cutaneous anaphylaxis (PCA) tests. The presence of proinsulin in calf serum has been demonstrated by (a) gel filtration of an acid alcohol extract of the serum followed by immunoassays of the fractions using anti-insulin or the specific anti-proinsulin serum, and (b) direct assay of calf serum with the specific anti-proinsulin serum.
منابع مشابه
The modified recombinant proinsulin: a simple and efficient route to produce insulin glargine in E. coli
Background: Recombinant insulin glargine, a long-acting analogue of insulin, is expressed as proinsulin in host cell and after purification and refolding steps cleaved to active insulin by enzymatic digestion using trypsin and carboxypeptidase B. Since the proinsulin's B and C chains have several internal arginine and lysine residues, a number of impurities are generated following treatment wit...
متن کاملPassive protective effect of anti-K99 antibodies against enterotoxigenic E.coli infection in neonatal calves
BACKGROUND: BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is the main causative agents of neonatal calf diarrhea. Passive transfer of antibodies that is derived from the dams of the calves through their colostrum plays an important role in disease prevention. OBJECTIVES: To determine the presence of natural specific antibodies against K99 antigen in dam’s serum and colostrum as well as in...
متن کاملEffects of n-3 and n-6 polyunsaturated fatty acids on the growth of fish cells in culture.
chromatography and liquid scintillation counting as described previously 141. Fig. 1 demonstrates a standard curve relating log,,, human proinsulin concentration t o specific activity of HMG-CoA reductase (pmol minI mgI protein). The human proinsulin concentration range for which this curve was linear was 2.7-2.7 x 1 0 5 PM. Thus, the detection limits of the bioassay presently include the physi...
متن کاملIdentification of proinsulin and C-peptide in human serum by a specific immunoassay.
An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of se...
متن کاملImmunoradiometric assay of human intact proinsulin applied to patients with type 2 diabetes, impaired glucose tolerance, and hyperandrogenism.
We describe an immunoradiometric assay for human intact proinsulin in serum. In this method, one monoclonal antibody, coated onto polyacrylamide beads, cross-reacts with proinsulins and insulin. A sandwich is formed with intact proinsulin, split (65-66) proinsulin, and des (64-65) proinsulin binding with an 125I-labeled monoclonal antibody specific for an epitope at the intact B-C junction of p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 62 2 شماره
صفحات -
تاریخ انتشار 1969