Secretion of newly synthesized proteins by isolated liver cells.
نویسندگان
چکیده
Suspensions of isolated liver cells were incubated with [3H]leucine as a precursor. The appearance of radioactive albumin and alpha 1-acid glycoprotein in the incubation medium was determined. After about 20 min both albumin and alpha 1-acid glycoprotein were secreted in a linear way. When fucose and galactose were used as radioactive precursors, time lags of about 10 min could be observed.
منابع مشابه
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Livers of normal mice were prefused in situ and the secretion of newly synthesized (i.e. labeled) proteins into the perfusate were measured. In control livers, the secretion of newly synthesized proteins was found to be linear with time. In marked contrast, when livers were perfused with vinblastine, vincristine, or colchicine, drugs known to interfere with the hepatic microtubular system, the ...
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عنوان ژورنال:
- Journal of cell science
دوره 42 شماره
صفحات -
تاریخ انتشار 1980