In vitro efficacy of cold atmospheric pressure plasma on S. sanguinis biofilms in comparison of two test models In vitro Wirksamkeit von kaltem Atmosphärendruckplasma gegen S. sanguinis Biofilme im Vergleich zweier Prüfmodelle

Dental plaque critically affects the etiology of caries, periodontitis and periimplantitis. Themechanical removal of plaque can only be performed Susanne Gorynia Ina Koban partially due to limited accessibility. Therefore, plaque still represents Rutger Matthes one of themajor therapeutic challenges. Even though antiseptic mouth Alexander Welk rinses reduce the extent of biofilm temporarily, plaque removal remains incomplete and continuous usage can even result in side effects. Sabine Gorynia Here we tested argon plasma produced by kinpen09 as one option to inactivatemicroorganisms and to eliminate plaque. S. sanguinis biofilms Nils-Olaf Hübner 1,4 Thomas Kocher cultivated in either the European Biofilm Reactor (EUREBI) or in 24 well plates were treated with argon plasma. In both test systems a homogenAxel Kramer eous, good analyzable and stable biofilm was produced on the surface of titan plates within 72 h (>6,9 log10 CFU/ml). Despite the significantly 1 Institute of Hygiene and Environmental Medicine, more powerful biofilm production in EUREBI, the difference of 0.4 log10 CFU/ml between EUREBI and the 24 well plates was practically not University Medicine relevant. For that reason both test models were equally qualified for the analysis of efficacy of cold atmospheric pressure plasma. Greifswald, Ernst-Moritz-Arndt University, Greifswald, Germany We demonstrate a significant reduction of the biofilm compared to the control in both test models. After plasma application of 180 s the biofilm 2 Department of Restorative Dentistry, Periodontology and produced in EUREBI or in 24 well plates was decreased by 0.6 log10 CFU/ml or 0.5 log10 CFU/ml, respectively. In comparison to recently Endodontology, University published studies analyzing the efficacy of kinpen09, S. sanguinis produces a hardly removable biofilm. Medicine Greifswald, ErnstMoritz-Arndt University Greifswald, Germany Future investigations using reduced distances between plasma source and biofilm, various compositions of plasma and alternative plasma 3 Oncotest GmBH, Freiburg, Germany sources will contribute to further optimization of the efficacy against S. sanguinis biofilms.