Feed Speed and Overfeed Volume New Parameters for Injection in Supercritical Fluid Chromatography
چکیده
This Technical Overview demonstrates and discusses the influence of the injection parameters Feed Speed and Overfeed Volume on chromatographic separation. These parameters were introduced with the Agilent 1260 Infinity II Supercritical Fluid Chromatography (SFC) Multisampler. The influence on the isocratic separation of closely eluting compounds, shown for different injection volumes commonly used in analytical SFC, and guidelines for optimization are given.