Enzymatic synthesis of oligosaccharides
نویسندگان
چکیده
The biological interest of oligosaccharides is growing very rapidly, and necessitates the development of efficient synthesis reactions. The stereoand regio-selectivity of enzyme catalysis is a key advantage in this field, as a complementary tool to the chemical approach. Two types of enzymes can be applied to the obtention of oligosaccharides: Hydrolytic enzymes, which can catalyze either reverse hydrolysis (thermodynamic control) or transglycosylation (kinetic control) synthesis reactions; and transferase enzymes, which can use simple carbohydrates from agricultural origin as glycosyl donors.
منابع مشابه
Optimization of Enzymatic Synthesis of Isomalto-oligosaccharides Production
Glucosyltransferases can be applied in the synthesis of prebiotic oligosaccharides. Enzymatic synthesis using acceptors can be used to obtain these carbohydrates. When maltose is the acceptor, oligosaccharides containing one maltose moiety and up to eight glucose units linked by a-1,6glycosidic bonds are obtained as the product of dextransucrase acceptor reaction. In this work, the enzymatic sy...
متن کاملThe mechanism of enzymatic synthesis of galactosyl oligosaccharides.
In recent publications, Wallenfels (1) and Aronson (2) have reported that new oligosaccharides are produced during the hydrolysis of lactose by enzyme preparations from bacteria and yeasts. The enzymatic synthesis of oligosaccharides from lactose has also been observed by Pazur (3) and by Roberts and McFarren (4). It has been suggested (l-3) that the new compounds are the result of transgalacto...
متن کاملCombination of automated solid-phase and enzymatic oligosaccharide synthesis provides access to α(2,3)-sialylated glycans.
A synthetic strategy combining automated solid-phase chemical synthesis and enzymatic sialylation was developed to access α(2,3)-sialylated glycans.
متن کاملDetailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases.
The production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2003