Diphenylamine reaction of serum in clinical medicine and chemistry.

Serum diphenylamine reactions were performed in 156 patients, medical and surgical. On the surgical side, serum diphenylamine reactions were evaluated in 50 patients before operation and on the 7th and 12th postoperative days. The diphenylamine reaction was revealed as a determination of serum sialo-mucoprotein level. The above cases justify the assumption that the serum diphenylamine reaction informs us of the intensity of regeneration in the organism. ∗PMID: 4263522 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 25, 205-215 (1971) DIPHENYLAMINE REACTION OF SERUM IN CLINICAL MEDICINE AND CHEMISTRY Gyorgy MEDGYESSY*, Endre SZIRMAI and Otto GUTTSCHES* * Department of Nuclear Hematology, The Institute of Nuclear Eng., London and Stuttgart (England and F. R. of Germany) Received for publication, January 27, 1971 Enormous progress has been made in our knowledge of sugar che. mistry during the past 50 years. However, chemical methods in this field are used relatively rarely and indeed very seldom in clinical laboratories. Bearing in mind that medical diagnostic institutions perform several routine examinations applied to lipids, e. g., total cholesterol, free chole. sterol, total fatty acids, esterified and free fatty acids, total lipids, triglycerides, lipid.electrophoresis, lipase activity, etc., it is remarkable that laboratories in large hospitals are not equipped for chemical examina. tions of sugars except, e. g., for determination of blood glucose. Again, when we consider the determination of amylase activity or glycoprotein electrophoresis or determination of fructose in urine or in spermatozoa, we have noted with regret that the possibilities offered by sugar chemistry are used very sparingly. It is probable that the modern documentation of experimental results is partly responsible for this situa. tion, in spite of the wide use of the computer and in part because of the often conflicting interpretation of results of researches in this field. It would seem that our somewhat rigid mental attitude inhibits the develop. ment of clinical sugar chemistry. 1. We often criticise the value of results reported by diagnostic laboratories when we consider them in relation to diagnosis of particular lesions and variations in the course of a disease. We often neglect existing methods which may be valuable for indicating improvement or convales. cence. With regard to improvement we should not only pay attention to the disappearance of pathological conditions, but should also ask ourselves the following questions : *) Karolt Institute for Medical Dia5nostics, Therapy and Research (Director: Dr. G. Medgyessy), Premerhaven, F. R. of Germany. **) Hospital Maria-Hilf, Ead Neuenahr (Physician-in-Chief: Dr. N. Nikolai), F. R. of Germany.