Screening and identification of a feruloyl esterase producing bacteria Burkholderia fungorum A216

A bacterial strain named A216, with feruloyl esterase producing capability, was isolated from forest soil after enrichment. The strain was identified at the species level by morphology, physiological-biochemical tests and 16S rRNA gene sequencing assay. The results revealed that strain A216 was accurately identified as Burkholderia fungorum, and the ITS sequence of the species was submitted to the NCBI for the first time. The enzyme activity in different medium was preliminarily analyzed by spectrophotometric assay. After incubation 5 days, it gave the maximal activity of feruloyl esterase 14.24 U/L when cultivated in fermentation medium. However, no feruloyl esterase activity was detected in LB medium under the same condition. To our best knowledge, this is the first observation of feruloyl esterase activity in the species Burkholderia fungorum.