Deletion of DNA sequence in a nononcogenic variant of Herpesvirus saimiri.
نویسندگان
چکیده
The 110-kilobase-pair stretch of unique sequence DNA of Herpesvirus saimiri is flanked by highly repetitive DNA. Detailed restriction endonuclease mapping has localized the left junction of repetitive and unique DNA to a 100-base-pair region. H. saimiri 11att, a replication competent nononcogenic variant of strain 11, has a deletion of 2.3 kilobase pairs of sequence information that spans this left junction of repetitive and unique DNA.
منابع مشابه
A role for herpesvirus saimiri orf14 in transformation and persistent infection.
The product of open reading frame 14 (orf14) of herpesvirus saimiri (HVS) exhibits significant homology with mouse mammary tumor virus superantigen. orf14 encodes a 50-kDa secreted glycoprotein, as shown previously (Z. Yao, E. Maraskovsky, M. K. Spriggs, J. I. Cohen, R. J. Armitage, and M. R. Alderson, J. Immunol. 156:3260-3266, 1996). orf14 expressed from recombinant baculovirus powerfully ind...
متن کاملMapping of RNA transcribed from a region of the Herpesvirus saimiri genome required for oncogenicity.
A region of the Herpesvirus saimiri genome that is not essential for replication of the virus has recently been shown to be required for its oncogenicity in New World primates. We have examined the RNAs derived from this region of the genome in permissively infected cells. Two polyadenylated RNAs, of 4.9 and 2.3 kilobases, were the major species coded for by this region of the genome. These two...
متن کاملSTP and Tip are essential for herpesvirus saimiri oncogenicity.
Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed. The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets. Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such ...
متن کاملtrans activation of the thymidylate synthase promoter of herpesvirus saimiri.
Herpesvirus saimiri has been shown to possess a thymidylate synthase (TS) gene that is unusual in its transcriptional regulation. Although TS is believed to be required for viral DNA synthesis, the TS-specific 2.5-kb mRNA was found most abundantly during the late phases of asynchronous virus replication in permissive cultures. To study the kinetics of gene activation, the TS promoter and regula...
متن کاملUse of virion DNA as a cloning vector for the construction of mutant and recombinant herpesviruses.
We have developed improved procedures for the isolation of deletion mutant, point mutant, and recombinant herpesvirus saimiri. These procedures take advantage of the absence of NotI and AscI restriction enzyme sites within the viral genome and use reporter genes for the identification of recombinant viruses. Genes for secreted engineered alkaline phosphatase and green fluorescent protein were p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of virology
دوره 50 2 شماره
صفحات -
تاریخ انتشار 1984