The Accumulation of Waxes by Acinetobacter calcoaceticus N.C.I.B. 8250

Constitutivity of the mandelate enzymes in Acinetobacter calcoaceticus, N.C.I.B. 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase.

Electron-Transport Systems Obtained from Vegetative Organisms and Spores of Bacillus megaterium KM

succinate contained increasing amounts of wax as the specific growth rate decreased from 0.7h-I to 0.12h-I. The wax composition also varied with dilution rate, samples having a high wax content had palmitic acid and palmityl alcohol as major components. In continuous culture with succinate limitation and excess of nitrogen, the bacteria contained only small amounts of waxes, which increased sli...

Solubilization studies and properties of particulate NAD-independent malate dehydrogenase of Acinetobacter calcoaceticus (N.C.I.B. 8250).

Erythrocytes from the same mouse strains gave similar results; here the cell number was increased to 109/ml to obtain a significant difference between curves for highand low-responder mice. It is possible that these results are affected by contamination with mouse ferritin from the spleen or plasma despite washing of cells. To minimize possible contamination by mouse ferritin, lymph-node cells,...

Two benzaldehyde dehydrogenases in bacterium N.C.I.B. 8250. Distinguishing properties and regulation.

Evidence is presented for the existence in bacterium N.C.I.B. 8250 of two inducible NAD(+)-linked benzaldehyde dehydrogenases. They may be distinguished in crude extracts by their different thermal stabilities at high pH values, benzaldehyde dehydrogenase I being much more heat-stable than benzaldehyde dehydrogenase II. Only benzaldehyde dehydrogenase I is activated by K(+) and certain other un...

Novel Oxadiazole Thioglycosides as Potential Anti- Acinetobacter Agents

The glycosylation of 1,3,4-oxadiazole-2-thiones has been performed with peracetylated β-pyranosyl bromide in the presence of potassium carbonate. Deprotection of acetylated thioglycosides was necessary for increasing their antibacterial effects. The structure of nucleosides were confirmed by 1H NMR, 13C NMR and HRMS. The anomeric protons of nucleosides c1–4 were assigned to the doublet, confirm...