Simultaneous spectrophotometric determination of diphenylhydantoin and phenobarbital in biologic specimens.
نویسنده
چکیده
A rapid, accurate method for determining diphenylhydantoinand phenobarbital in a single biologic sample is described. Separation of the two drugs after their initial chloroform extraction from the specimen is not required. After extraction, diphenylhydantoin is converted to benzophenone by pemanganate oxidation. Phenobarbital is determined by differential absorption between pH values of 12.0 and 10.5. Final quantitation in each case is carried out by ultraviolet spectrophotometry. Barbiturates do not interfere with the diphenyihydantoin analysis or vice versa. The method is sensitive enough for detecting subtherapeutic amounts of each compound with a high degree of specificity.
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عنوان ژورنال:
- Clinical chemistry
دوره 15 4 شماره
صفحات -
تاریخ انتشار 1969