Polaprezinc prevents ongoing thioacetamide-induced liver fibrosis in rats.

نویسندگان

  • Toru Kono
  • Toshiyuki Asama
  • Naoyuki Chisato
  • Yoshiaki Ebisawa
  • Taishi Okayama
  • Kouji Imai
  • Hidenori Karasaki
  • Hiroyuki Furukawa
  • Masashi Yoneda
چکیده

AIMS Cirrhotic patients commonly have a liver zinc deficiency, which may aggravate liver fibrosis due to the lack of antioxidative effects of zinc. This study examined the ability of polaprezinc, N-(3-aminopropionyl)-l-histidinato zinc, to prevent fibrosis in a rat model of thioacetamide (TAA)-induced hepatic fibrosis. MAIN METHODS Liver cirrhosis was induced by orally administering TAA for 20 weeks. The rats were cotreated with one of the following for the last 10 weeks of TAA treatment: (1) polaprezinc (50 or 200mg/kg/day); (2) l-carnosine (155 mg/kg/day), which contained equal amounts of l-carnosine as 200mg/kg/day polaprezinc; (3) zinc sulfate (112 mg/kg/day) or (4) zinc-l-aspartic complex (317.8 mg/kg/day). Both zinc supplementations contained equal amounts of zinc as high-dose polaprezinc. KEY FINDINGS Hepatic zinc levels fell significantly in rats treated with TAA for 20 weeks. Cotreating with high-dose polaprezinc and zinc-l-aspartic complex for 10 weeks prevented hepatic zinc loss. Hepatic hydroxyproline and tissue inhibitor of metalloproteinases-1 (TIMP-1) were significantly higher in rats treated with TAA for 20 weeks than 10 weeks, whereas polaprezinc prevented changes in these fibrosis markers and reduced hepatic transforming growth factor-β1 protein concentration, macroscopic and histologic changes. TAA caused oxidative stress-related changes in the liver that were prevented by high-dose polaprezinc and partially by zinc-l-aspartic complex. Treatment with l-carnosine, low-dose polaprezinc or zinc sulfate for 10 weeks did not affect liver fibrosis progression or oxidative stress-related changes. SIGNIFICANCE Polaprezinc may prevent ongoing fibrosis by preventing zinc depletion, oxidative stress and fibrosis markers in cirrhotic livers.

برای دسترسی به متن کامل این مقاله و 10 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats

The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of th...

متن کامل

Protective Effects of Extract from Dates (Phoenix Dactylifera L.) and Ascorbic Acid on Thioacetamide-Induced Hepatotoxicity in Rats

The ameliorative activity of aqueous extract of the flesh of dates (Phoenix dactylifera L.) and ascorbic acid on thioacetamide-induced hepatotoxicity was studied in rats. Sixty male rats were divided into six equal groups of 10. Two groups were controls, one treated with thioacetamide and one with only distilled water. Two groups received extract of flesh Phoenix dactylifera and intraperitoneal...

متن کامل

Protection of rats from thioacetamide-induced hepatic fibrosis by the extracts of a traditional Uighur medicine Cichorium glandulosum

Objective(s):To clarify the protective effects of Cichorium glandulosum (CG) extracts on thioacetamide (TAA)-induced rat hepatic fibrosis. Materials and Methods: The dry roots of CG were smashed and percolated with 95% ethanol, and the residual was prepared into petroleum ether extract (CG-V), ethyl acetate extract (CG-VI) and n-butyl alcohol extract (CG-VII). Thirty-six Wistar rats were random...

متن کامل

A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats

 Background and purpose: This study compared the effects of intraperitoneal injections of ZnO/chitosan nanocomposite and ZnO nanoparticles on histopathology of kidney in thioacetamide-intoxicated rats. Materials and methods: Twenty-eight male rats were randomly allocated into a healthy control group (negative control), positive control group (thioacetamide-intoxicated rats), thioacetamide-into...

متن کامل

Hepatoprotective Role of Ethanolic Extract of Vitex negundo in Thioacetamide-Induced Liver Fibrosis in Male Rats

The hepatoprotective activity of ethanolic extract from the leaves of Vitex negundo (VN) was conducted against thioacetamide- (TAA-) induced hepatic injury in Sprague Dawley rats. The therapeutic effect of the extract was investigated on adult male rats. Rats were divided into seven groups: control, TAA, Silymarin (SY), and VN high dose and low dose groups. Rats were administered with VN extrac...

متن کامل

افزودن به منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی راحت تر خواهید کرد

برای دسترسی به متن کامل این مقاله و 10 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Life sciences

دوره 90 3-4  شماره 

صفحات  -

تاریخ انتشار 2012