Genome Sequence of Bluetongue Virus Type 2 from India: Evidence for Reassortment between Outer Capsid Protein Genes

Cloning of Rota Virus Outer Capsid Protein (VP7) Gene into the pGEM Vector

Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

Genetic Characterization of Toggenburg Orbivirus, a New Bluetongue Virus, from Goats, Switzerland

A novel bluetongue virus (BTV) termed Toggenburg orbivirus (TOV) was detected in goats from Switzerland by using real-time reverse transcription-PCR. cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplified by PCR and cloned into a plasmid vector. Five clones for each genome segment were sequenced to determine a consensus sequence. BL...

Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes.

The outer capsid protein VP2 of Bluetongue virus (BTV) is a target for the protective immune response generated by the mammalian host. VP2 contains the majority of epitopes that are recognized by neutralizing antibodies and is therefore also the primary determinant of BTV serotype. Full-length cDNA copies of genome segment 2 (Seg-2, which encodes VP2) from the reference strains of each of the 2...

Complete sequence of VP2 gene of the bluetongue virus serotype 1 (BTV-1).

A clone representing a full length cDNA copy of the bluetongue virus (BTV) outer capsid protein VP2 gene (serotype 1, isolated in Australia) was obtained using methods described elsewhere (1). The complete sequence of the gene determined by dideoxy method (2) was deduced to be 2940 base pairs long. It codes for a primary gene product of 962 amino acids with an estimated M.W. 112,087, a comparab...

Nucleotide Sequence of Gene Encoding Capsid Protein VPI of Foot-and-Mouth Disease Virus / Type O1 Iran