Allelic Variation of VRN-1 Locus in Iranian Wheat Landraces

Authors

  • Behnam Derakhshani Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran
  • Mohammad Moghaddam 1Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran 2Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz 51666, Iran
  • Mohammad Reza Jalal Kamali CIMMYT (International Maize and Wheat Improvement Center), Shahid Fahmideh Blvd., Karaj 31585, Iran
  • Seyed Abolghasem Mohammadi Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran. 2Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz 51666, Iran
Abstract:

Wheat is a crop with spring and winter types and wide adaptability to different climate conditions. The wide adaptability of wheat is mainly controlled by three groups of genetic factors and among them vernalization (VRN) genes play pivotal role in determining spring and winter types. In this study, 395 Iranian wheat landraces were characterized with specific primer pairs designed based on VRN-1 promoter and intron regions. Using the specific primers for Vrn-A1c allele, two fragments were amplified in 35 genotypes. Based on MADS-Box and promoter regions of VRN-1 gene specific primers, two new fragments were amplified in Iranian wheat landraces which has not been reported previously. Vrn-A1b allele determining spring habit was the most frequent allele, whereas Vrn-A1c showed less frequency. Frequency of dominant allele Vrn-A1b, in winter genotypes was higher than that of spring type. It supports the presence of other regulatory sites outside of the VRN promoter region.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

allelic variation of vrn-1 locus in iranian wheat landraces

wheat is a crop with spring and winter types and wide adaptability to different climate conditions. the wide adaptability of wheat is mainly controlled by three groups of genetic factors and among them vernalization (vrn) genes play pivotal role in determining spring and winter types. in this study, 395 iranian wheat landraces were characterized with specific primer pairs designed based on vrn-...

full text

Genetic Variation Pattern of Iranian Wheat Landraces Based on ISSR Molecular Markers and Morphological Traits

Collection and conservation of germplasm, particularly for landraces of important agronomically species and organizing their information, cause to continuity of their usefulness and reduce the risk of their genetic erosion. In this experiment, 10 Iranian wheat landraces along with the Chinese Spring cultivar, as control, were used to assess inter and intra populations variation by using ISSR mo...

full text

Genetic Linkage Map of Glu-D1 and Allelic Variation of HMW Glutenin Subunits in Some Iranian Bread Wheat genotypes

High-molecular weight (HMW (glutenin subunits are encoded by the Glu-1 loci (Glu-A1, Glu-B1 and <sp...

full text

PCR-based markers for identification of some allelic variation at Glu-1 and Glu-3 loci in common wheat

Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars...

full text

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results ...

full text

Allelic Variation at the Rht8 Locus in a 19th Century Wheat Collection

Wheat breeding during the 20th century has put large efforts into reducing straw length and increasing harvest index. In the 1920s an allele of Rht8 with dwarfing effects, found in the Japanese cultivar "Akakomugi," was bred into European cultivars and subsequently spread over the world. Rht8 has not been cloned, but the microsatellite marker WMS261 has been shown to be closely linked to it and...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 3  issue 1

pages  45- 56

publication date 2013-04-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023