Association mapping for resistance to powdery mildew in oriental tobacco (Nicotiana tabaccum L.) germplasm

Authors

Abstract:

Powdery mildew caused by Erysiphe cichoracearum is an important fungal disease which threatens tobacco (Nicotiana tabacum L.) production. The objective of this study was to determine DNA markers linked to genomic regions associated with resistance to powdery mildew in tobacco through the association mapping approach. Seventy tobacco geno-types were fingerprinted using 26 simple se-quence repeat (SSR) primer pairs which were distributed in several chromosomes of tobac-co. A total number of 66 alleles were detected by 26 SSR primers with an average of 2.53 alleles per locus. Based on population struc-ture analysis, the studied genotypes were classified into three groups including Chopogh, PD and Tikolak genotypes. Ou of 325 marker pairs, 6.15% showed a significant level of linkage disequilibrium (LD) (P

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Journal title

volume 3  issue 1

pages  30- 21

publication date 2014-04-01

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