Effects of Diamond nanoparticles on mitochondrial apoptosis pathway in rats with testicular ischemia / reperfusion.

Authors

  • Angaji, Seyed Abdolhamid Department of Cell and Molecular Biology, Faculty of biosciences, ,Kharazmi university, Tehran, Iran
  • Hashemi , Mehrdad Department of Genetics, faculty of Advanced science and Technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran ----Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Masoumi, Masoumeh Department of Genetics, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
  • Salehi, Mitra Department of Microbiology, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
Abstract:

Background and Aim: Testicular torsion is a urological emergency that leads to testicular tissue damage and infertility. In recent years, several studies have been conducted on the antioxidant properties of nanoparticles. The aim of this study was to investigate the effects of Diamond nanoparticles on the  apoptosis in rats with testicular ischemia / reperfusion. Methods: 48 adult rats aged 20 weeks and weighing 250 g in the control and ischemia / testicular reperfusion groups were included in the study. Both groups were treated with q10 and nanodiamond at a dose of LD50 for 10 days. Then the testes were removed and the survival and apoptosis of the cells were assessed by MTT and flowcytometry and the expression of BAX and BCL2 genes was measured. Results: Treatment with nanodiamond in the control group increased the expression of BAX gene and decreased the expression of BCL2 gene, while in the ischemia / reperfusion group it had the opposite effect. Simultaneous use of Q10 and nanodiamond compensated for the adverse effects of nanodiamond (P <0.05). Conclusion: Nanodiamond at LD50 dose in rats with testicular ischemia / reperfusion had antioxidant effects and reduced apoptosis.  

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Simultaneous effects of curcumin and Fe3o4 nanoparticles on expression of Bad and Bclx genes in mitochondrial apoptosis in rats with testicular ischemia

Background and Aims: Testicular ischemia / reperfusion is one of the most serious urological emergencies that is generally identified in men, adolescents and infants. This causes damage to the testicles which, if misdiagnosed and treated improperly, can lead to infertility in men. In the present study, the effects of curcumin and Fe3O4 nanoparticles on the rate of apoptosis and expression of BA...

full text

Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats

This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, ...

full text

Effects of Echinacea Purpurae Extract on Testicular Ischemia/Reperfusion (I/R) Injury in Rat

BACKGROUND: Ischemia/reperfusion of tetsis is a male infertility condition which occurs because of oxidation demage. Echinacea purpurea extract (EPE) has antioxidant and protective effect. OBJECTIVES: So, the main purpose of this research was to determine effects of EP extract on testicular ischemia/reperfusion (I/R) injury in rat. METHODS: 50 adult Wistar rats were randomly allocated into five...

full text

effects of ghrelin on testicular ischemia/reperfusion-induced injury

ischemia-reperfusion injury is a possible cause of testicular damage and infertility after testicular torsion and detorsion. the purpose of this study was to evaluate the effect of ghrelin on testicular ischemia-reperfusion damage. a total of 30 adult male rats were selected for the study and divided randomly into 3 groups, each containing 10 rats. animals in the testicular torsion and ghrelin ...

full text

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats.

PURPOSE To determine the effect of folic acid (FA) on experimental testicular ischemia/reperfusion (I/R) in rats. METHODS Sixty male Wistar rats were randomly divided into 6 groups. The control group received physiologic saline orally. The sham-operated group received physiologic saline orally then exposed to midline laparotomy without clamping the IR. The I/R rats received oral gavage of the...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 0  issue None

pages  0- 0

publication date 2021-12

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023