Mohammad Reza Fazeli

[ 1 ] - Optimization of Culture Conditions for Enrichment of Saccharomyces cerevisiae with Dl-α-Tocopherol by Response Surface Methodology

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with α-tocopherol produced as an accumulator and transporter of a lipid soluble vitamin for the fir...

[ 2 ] - Nonionic Surfactants (Dodecyl Maltoside and Polysorbate 20) Effect on Light induced Aggregation and Conformational Changes of Recombinant Human IFNβ_1b

Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its...

[ 3 ] - Development and Validation of a Micellar Capillary Electrophoresis Method for Determination of IFNβ-1b in Lyophilized Formulation of a Biosimilar Product

Human interferons (IFNs) are key cytokines secreted by immune system. They have several effects such as antiviral and anti tumors activity, activating immune cells and healing of multiple sclerosis. The type IFNs present in humans are α, β and Υ. IFN β is a polypeptide, normally produced by fibroblasts and seems to be more species-specific than IFN . Structural properties of I FNs are importan...

[ 4 ] - Evaluation of Thimerosal Removal on Immunogenicity of Aluminum Salts Adjuvanted Recombinant Hepatitis B Vaccine

Thimerosal, which is approximately 50% mercury by weight is a preservative widely used in vaccines since the 1930’s. It meets the requirements for a preservative as set forth by Pharmacopeia challenge test and has been shown to be effective against a broad spectrum of pathogens. In July 1999, the Public Health Service agencies and vaccine manufacturers agreed that thimerosal should be redu...

[ 5 ] - Optimization of Culture Conditions for Enrichment of Saccharomyces cerevisiae with Dl-α-Tocopherol by Response Surface Methodology

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with α-tocopherol produced as an accumulator and transporter of a lipid soluble vitamin for the fir...

[ 6 ] - Nonionic Surfactants (Dodecyl Maltoside and Polysorbate 20) Effect on Light induced Aggregation and Conformational Changes of Recombinant Human IFNβ_1b

Liquid protein formulations are prone to form aggregates. The effect of nonionic surfactants such as Polysorbate 20 (PS 20) and n-Dodecyl β-D-maltoside (DDM) on the prevention of aggregation and conformational changes of recombinant human IFNβ-1b (rhIFN β_1b) was explored. Polysorbate has been used in formulations of protein pharmaceuticals. There have been concerns about using PS 20 due to its...

[ 7 ] - Development and Validation of a Micellar Capillary Electrophoresis Method for Determination of IFNβ-1b in Lyophilized Formulation of a Biosimilar Product

Human interferons (IFNs) are key cytokines secreted by immune system. They have several effects such as antiviral and anti tumors activity, activating immune cells and healing of multiple sclerosis. The type IFNs present in humans are α, β and Υ. IFN β is a polypeptide, normally produced by fibroblasts and seems to be more species-specific than IFN . Structural properties of I FNs are importan...

[ 8 ] - Evaluation of Thimerosal Removal on Immunogenicity of Aluminum Salts Adjuvanted Recombinant Hepatitis B Vaccine

Thimerosal, which is approximately 50% mercury by weight is a preservative widely used in vaccines since the 1930’s. It meets the requirements for a preservative as set forth by Pharmacopeia challenge test and has been shown to be effective against a broad spectrum of pathogens. In July 1999, the Public Health Service agencies and vaccine manufacturers agreed that thimerosal should be redu...

[ 9 ] - Production of vitamin D3 enriched biomass of Saccharomyces cerevisiae as a potential food supplement: evaluation and optimization of culture conditions using Plackett–Burman and response surface methodological approaches

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this st...

[ 10 ] - Production of vitamin D3 enriched biomass of Saccharomyces cerevisiae as a potential food supplement: evaluation and optimization of culture conditions using Plackett–Burman and response surface methodological approaches

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this st...

[ 11 ] - A Simplified Process for Purification and Refolding of Recombinant Human Interferon-α2b

Background: Interferon α-2b is a vital biotherapeutic produced through the recombinant DNA technology in E. coli. The recombinant IFN-α2b normally appears as intercellular IBs, which requires intensive refolding and purification steps. Method: Purification of IFN-α2b from solubilized IB was performed using two-phase extraction. To optimize refolding conditions, the effects of pH and different a...