اثر ضد باکتریائی بره موم زنبور عسل روی تعدادی از باکتریهای بیماریزا

ABSTRACT Propolis or bee glue is a production of hive with resine nature which had been known from ancient times and was used in treatment of infectious disease. At present time it is still widely used in the eastern parts of Europe. The use of propolis for its antibacterial, antiviral, antifungal and certain other useful pharmacologic effects is increasing. Because of difference between the sort of propolis from various countries, we decided to investigate the propolis of a specific part of Iran. To do this, the antibacterial effect of Borougerd city propolis was evaluated. In this study, ethanol (70%), methanol and chloroform extracted from propolis on the concentrations of 30, 20, 10, 5, 2.5 and 1.25% W/V were prepared by digestion method. Paper disk method was used for evaluation of the antibacterial effect of the extracts because of the advantages claimed for this method. To prepare the disk, ten microliter of the above mentioned concentrations were added to the blank disks, so after drying. the disks contained 3, 2, 1, 0.25 and 0.125 miligram of each extract. The effect of the extracts were then investigated on the S. aureus, Strep, pyogenes, Pseudomonas aeruginosa, Sal. typhi and proteus vulgaris cultured on muller- Hinton agar. The inhibition zones produced by the disks were assessed after 18 to 24 hours. According to the results obtained, it was concluded that only S. aureus and Strep. pyogenes arc sensitive to the propolis extract and other strains of the bacteria are resistant to it. It was also shown that propolis extracts in the above mentioned concentrations have inhibitory effect on the growth of the sensitive bacteria. There was not any significant difference betwecn ethanol, methanol or chloroform extracts, so it suggests that the extracting solvent does not have any influence on the antibacterial effect. For statistical evaluation, analysis of variance and tukey tests were used.