افزایش تولید نایسین در بیوراکتور با کشت همزمان Lactococcus lactis و Yarrowia lipolytica

Yarrowia lipolytica in Biotechnological Applications

The nonconventional yeast Yarrowia lipolytica has been developed as a versatile and attractive tool for a large variety of biotechnological applications. This yeast has several physiological properties with industrial significance. Y. lipolytica uses hydrophobic substrates such as n-alkanes, oils, fats, and fatty acids as low-cost carbon sources. The yeast is able to produce a set of diverse ad...

The Complete Mitochondrial Genome of Yarrowia Lipolytica

We here report the complete nucleotide sequence of the 47.9 kb mitochondrial (mt) genome from the obligate aerobic yeast Yarrowia lipolytica. It encodes, all on the same strand, seven subunits of NADH: ubiquinone oxidoreductase (ND1-6, ND4L), apocytochrome b (COB), three subunits of cytochrome oxidase (COX1, 2, 3), three subunits of ATP synthetase (ATP6, 8 and 9), small and large ribosomal RNAs...

Engineering Yarrowia lipolytica for Campesterol Overproduction

Campesterol is an important precursor for many sterol drugs, e.g. progesterone and hydrocortisone. In order to produce campesterol in Yarrowia lipolytica, C-22 desaturase encoding gene ERG5 was disrupted and the heterologous 7-dehydrocholesterol reductase (DHCR7) encoding gene was constitutively expressed. The codon-optimized DHCR7 from Rallus norvegicus, Oryza saliva and Xenapus laevis were ex...

A molecular genetic toolbox for Yarrowia lipolytica

BACKGROUND Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain. RESULTS We have developed a set of genetic and molecular tools in order to expand capabilities of Y. li...

Instability in Lactococcus lactis

Lactococcus lactis as a live delivery vector

Mucosal surfaces of the body provide a universal entry portal for all known and emerging infectious pathogenic microbes. Therefore, it seems that special vaccination strategies are needed for vaccines that can hinder the entry capability of pathogenic microbes through the mucosal surfaces. Lactic acid bacteria are widely used in the food industry and are presently applied as delivery vehicles i...