مقایسه روش‌های نگهداری فراسرد ژرم پلاسم گیاهی بر شاخص‌های رشدی و جوانه‌زنی بذر گیاه آویشن دنایی

Increasing rate of extinction of valuable plant species such as Thymus daenensis Celak due to various biological and non-biological factors requires an overview of conservation and preservation methods. In this regard, cryopreservation techniques are one of the most important methods for preservation of seeds in non-habitat conditions. In the present study, the effects of different plant vitrification solutions (PVS2 and PVS3) were evaluated using two methods of vitrification (encapsulation-vitrification and droplet-vitrification) on the longitudinal and germination indices of T. daenensis seeds. Results showed that the highest viability rate (98%) and seed vigor index (26) was obtained after droplet-vitrification method using PVS3 (p ≤0.05). Also, the lowest seedlings height was observed in encapsulation-vitrification method using PVS2 (17/33 mm; p ≤0.05). Therefore, droplet-vitrification method using PVS3 is more favorable for the long-term preservation of T. daenensis seeds.