Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus

نویسندگان

  • Abbasali Eskandarian Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Ali Moosavi Iran University of Medical Sciences, Tehran, Iran.
  • Alireza Hejrati Iran University of Medical Sciences, Tehran, Iran.
  • Hossein Yousofi Darani Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Lame Akhlaghi Department of Medical Parasitology and Mycology, Faculty of Medicine, Iran university of Medical Sciences, Tehran, Iran.
  • Raheleh Rafiei Sefiddashti Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Seyedeh Maryam Sharafi Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Soltan Ahmad Ebrahimi Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
چکیده مقاله:

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst fluid (CF) and protoscolex (PS) antigens of E. Granulosus using ELISA and western immunoblotting tests. Thin-layer chromatography and ß-elimination were used for glycan purification. Results: Both hydatid cyst and normal human sera reacted with hydatid cyst fluid, protoscolices, laminated layer, glycoprotein and glycolipid antigens. The most antigen-antibody reaction was related to CF and PS antigens, and LL antigens had the minimal reaction with the sera. Thin layer chromatography (TLC) of the antigens showed presence of many glycan bands in the laminated layer. Conclusion: The parasite may elaborate different glycan antigens in LL to evade host immune response.

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Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst...

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عنوان ژورنال

دوره 31  شماره 1

صفحات  66- 70

تاریخ انتشار 2017-01

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