Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

نویسندگان: ثبت نشده
چکیده مقاله:

این مقاله چکیده ندارد

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

cloning and expression of recombinant coat protein gene of turnip curly top virus in escherichia coli

turnip curly top virus (tctv) is a member of the newly established genus turncurtovirus, family geminiviridae. development of an efficient detection system is necessary to study the biological characteristics of viruses. due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of t...

متن کامل

Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli

Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

متن کامل

Cloning and expression of fragment of the rabies virus nucleoprotein gene in Escherichia coli and evaluation of antigenicity of the expression product

Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long...

متن کامل

Local and distant sequences are required for efficient readthrough of the barley yellow dwarf virus PAV coat protein gene stop codon.

Many viruses use stop codon readthrough as a strategy to produce extended coat or replicase proteins. The stop codon of the barley yellow dwarf virus (PAV serotype) coat protein gene is read through at a low rate. This produces an extended polypeptide which becomes part of the virion. We have analyzed the cis-acting sequences in the barley yellow dwarf virus PAV genome required for this program...

متن کامل

expression of citrus tristeza virus coat protein gene in escherichia coli

citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...

متن کامل

Molecular and Biological Characterization of a Trackable Illinois Isolate of Barley yellow dwarf virus-PAV

Jae Sun Moon and Richard G. Allen, Department of Crop Sciences, University of Illinois, 1102 South Goodwin Ave., Urbana 61801; Leslie L. Domier, Department of Crop Sciences, University of Illinois, 1102 South Goodwin Ave., Urbana 61801 and U.S. Department of Agriculture, Agricultural Research Service, Crop Protection Research Unit, 1102 South Goodwin Ave., Urbana, IL 61801; and Adriana D. Hewin...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 2  شماره 2

صفحات  84- 89

تاریخ انتشار 2004-04-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023