Construction, Expression and Preliminary Immunological Evaluation of a DNA Plasmid Encoding the GRA2 Protein of Toxoplasma gondii

construction, expression and preliminary immunological evaluation of a dna plasmid encoding the gra2 protein of toxoplasma gondii

toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. the infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. dna vaccination may be a powerful approach against intracellular parasites such as toxoplasma gondii. the goal of this study was t...

High-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein

construction and in vitro expression analyses of a dna plasmid encoding dense granule gra5 antigen of toxoplasma gondii

toxoplasmosis is an infection caused by the protozoan parasite toxoplasma gondii (t.gondii) throughout the world. although usually asymptomatic, the infection can cause serious medical problems in immunocompromised individuals and fetuses. toxoplasmosis also causes considerable economic loss because of abortion in livestock. dna vaccination is a promising approach against intracellular parasite...

Construction of an Expression Plasmid (Vector) Encoding Brucella melitensis Outer Membrane Protein, a Candidate for DNA Vaccine

Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a pla...

Construction and In vitro Expression Analyses of a DNA Plasmid Encoding Dense Granule GRA5 Antigen of Toxoplasma gondii

Toxoplasmosis is an infection caused by the protozoan parasite Toxoplasma gondii (T.gondii) throughout the world. Although usually asymptomatic, the infection can cause serious medical problems in immunocompromised individuals and fetuses. Toxoplasmosis also causes considerable economic loss because of abortion in livestock. DNA vaccination is a promising approach against intracellular parasite...

Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine

Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...