Effect of Two Polarized Culture System Prepared From Human Female Genital Tract on Mouse Embryo Development
Purpose: To compare effects of polarized epithelial monolayer prepared from human oviduct and uterus on mouse one and two-cell embryo development. Materials and Methods: Human oviduct and endometrial tissue was obtained from patients who had undergone total hysterectomy. The epithelial cells were isolated from tissue and cultured on extracellular matrix (ECM) Gel coated Millipore filter insert under polarizing condition. The polarized state of cultured cells were evaluated by transmission electron microscopy (TEM).Two cell embryo of superovulated NMRI mouse were then flused and cultured either with oviduct or uterine polarized cell and medium alone. The rate of development in all groups were daily determined and statistically compared. At the end of cultivation period, some blastocyst from each group were differentially stained and mean cell number were counted and statistically compared. Results: Presence of tight junction between the cultured cells was evidenced in sections and confirmed polarized state of both kind of epitheial cells. These cells were columnar in shape. According to co-culture results, high precentage of one cell embryos overcame. developmental block and reached to blastocyst stage in both polarized groups as compared to control. Cell counting result showed that blastocyst's co-culture groups had more blastomere than that of control. Similarly two cell embryos in polarized groups showed increased development. All the observed differences showed statisticaly significant correlations. In polarized oviduct groups versus uterine, percenage of the embryos that overcame developmental block, blastocyst formation rate and the blastocyst cellularity were high and in uterine polarized groups, more blastocyst reached to hatching stage. Above mentioned differences between two polarized groups were not statistically significant. Conclusion: Both kind of polarized epithelial cells are significantly useful for mouse embryos development, but in this term there is no significant difference between oviduct and uterine cells. Although more percentage of embryos reach to higher stage (hatching blastocyst) when being cultured on uterine polarized group.
effect of polarized human uterine epithelial cells on mouse embryo development and blastocyst cellularity
introduction: the aim of this study was to investigate the growth and development of mouse two cell embryo on human uterine polarized monolayers. material and methodes: human endometrial tissue was obtained from patients who had undergone total hysterectomy. then, epithelial cells were isolated by collagenase i 0.25% and cultured on either ecm gel coated millipore filter insert (polarized) or p...متن کامل
this experimental study has been conducted to test the effect of oral presentation on the development of l2 learners grammar. but this oral presentation is not merely a deductive instruction of grammatical points, in this presentation two hypotheses of krashen (input and low filter hypotheses), stevicks viewpoints on grammar explanation and correction and widdowsons opinion on limited use of l1...15 صفحه اول
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...متن کامل
A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development
BACKGROUND: Co-culture of embryos with various somatic cells has been suggested as a promising approach to improve embryo development. Despite numerous reports regarding the beneficial effects of epithelial cells from the female genital tract on embryo development in a co-culture system, little is known about the effect of these cells when being cultured under a polarized condition on embryo gr...متن کامل
Effect of LH Treated Ovine Oviductal Epithelial Cell Co-Culture System on Murine Pre-Embryo Development
Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...متن کامل
Environmental oestrogens have been implicated in the pathogenesis of hormonally treated cancers (such as breast and prostate cancer), male infertility, and abnormalities of the male and female reproductive tracts. They may be derived from plants (phytoestrogens), pharmaceuticals, or other synthetic compounds not originally intended to have oestrogenic activity (including soy based infant formul...متن کامل
دوره 3 شماره None
صفحات 83- 92
تاریخ انتشار 2005-07
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