Extractive Spectrophotometric Determination of Miconazole Nitrate in Pure and Pharmaceutical Preparation

A simple extractive spectrophotometric method has been developed for the estimation of miconazole nitratein both pure and pharmaceutical preparation. The method is based on the formation of ion-pair complex of thedrug with acidic dye bromothymol blue (BTB) in acidic condition, followed by its extraction in organic solvent(chloroform). The absorbance was measured against the corresponding blank. The maximum absorbancewas found at 434 nm. Linear calibration graph was obtained in the concentration range 1-35 μg ml-1. Themethod was validated statistically. Recovery studies gave satisfactory results indicating that none of commonadditives and excipients interfere the assay method. The proposed methods were found to be simple, accurate nd reproducible and their performance in analysis of cream formulation was successfully examined.