Genetic Analysis of Hemagglutinin Protein of H9N2 Isolated from Live Bird Markets in Tehran Province in 2007-2008

نویسندگان

  • M Tavassoti-Kheiri Influenza Unit, Pasteur Institute of Iran, Tehran, Iran
  • MH Rafiei Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • MH Bozorgmehri-Fard Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • S Ghadi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • V Karimi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده مقاله:

Background and Aims: Influenza A virus subtype H9N2 have continued to circulate in domestic poultry farms in Asia since 1998. The virus circulates in live bird markets, missing link in epidemiology of avian influenza. Regarding previous studies on H9N2 viruses of Iran and having no data on this subject in Iranian live bird markets this study was conducted to analyze genetically hemagglutinin protein of H9N1 virus. Methods: A total of 500 tracheal and cloacal swabs from clinically healthy birds of Tehran's live bird markets were collected. Diagnostic RT-PCR was done on them using specific primers for subtype H9N2. Eight positive samples were selected for inoculation into 9-11 days SPF emberyonated eggs and the virus was grown and isolated. Amplification of the HA gene was carried out by PCR using two pairs of specific primers. PCR products were separated, purified and cloned. The products were sequenced and analyzed with M13 primers. They shared high amino acid homology with genes of other H9N2 viruses isolated previously in Asia and Iran. Results: H9N2 viruses isolated from live bird markets were highly similar to viruses isolated from industrial poultry being circulated as early as 2001. Conclusion: The results suggest that a common source of H9N2 viruses is circulating in Iran.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

genetic analysis of hemagglutinin protein of h9n2 isolated from live bird markets in tehran province in 2007-2008

background and aims: influenza a virus subtype h9n2 have continued to circulate in domestic poultry farms in asia since 1998. the virus circulates in live bird markets, missing link in epidemiology of avian influenza. regarding previous studies on h9n2 viruses of iran and having no data on this subject in iranian live bird markets this study was conducted to analyze genetically hemagglutinin pr...

متن کامل

Amino Acid Sequence Analysis of Hemagglutinin Protein of H9N2 Isolated from Broilers in Tehran in 2007

Background and Aims: Since 1998, Iranian poultry industry has been affected by avian influenza (AI) virus, subtype H9N2. The association of high mortality and case report of H5N1 and H9N2 influenza virus in wild birds in recent years raised the suspicion of a possible new genetic modified AI virus. Methods: Partial nucleotide sequences and deduced amino acid of hemagglutinin (HA) genes of 4 H9...

متن کامل

analysis of power in the network society

اندیشمندان و صاحب نظران علوم اجتماعی بر این باورند که مرحله تازه ای در تاریخ جوامع بشری اغاز شده است. ویژگیهای این جامعه نو را می توان پدیده هایی از جمله اقتصاد اطلاعاتی جهانی ، هندسه متغیر شبکه ای، فرهنگ مجاز واقعی ، توسعه حیرت انگیز فناوری های دیجیتال، خدمات پیوسته و نیز فشردگی زمان و مکان برشمرد. از سوی دیگر قدرت به عنوان موضوع اصلی علم سیاست جایگاه مهمی در روابط انسانی دارد، قدرت و بازتولید...

15 صفحه اول

this study investigated the relationship between quality of work life and organization citizenship behavior from the viewpoint of teachers in high schools in tehran city.

چکیده هدف از پژوهش حاضر بررسی رابطه کیفیت زندگی کاری و رفتار شهروندی سازمانی در مدارس مقطع متوسطه پسرانه شهر تهران از دیدگاه دبیران بود. روش پژوهش حاضر توصیفی و از نوع همبستگی بود. از بین دبیران مدارس شهر تهران تعداد 380 نفر به روش نمونه گیری خوشه ای چند مرحله ای به عنوان نمونه پژوهش انتخاب شدند و با تکمیل پرسشنامه ی کیفیت زندگی کاری والتون(1975) و پرسشنامه ی رفتار شهروندی سازمانی پودساکو...

15 صفحه اول

Genetic and molecular characterization of H9N2 and H5 avian influenza viruses from live poultry markets in Zhejiang Province, eastern China

Live poultry markets (LPMs) are a key source of reassorted avian influenza viruses (AIVs) because of the density of terrestrial and aquatic poultry and the frequency of AIV infection. H9N2 viruses are prevalent in terrestrial poultry throughout Asia and have been isolated from poultry outbreaks worldwide. They infect both avian and mammalian species and may be significant donors of genetic mate...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 3  شماره None

صفحات  7- 15

تاریخ انتشار 2009-10

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023