Identification of virulence genes in Pseudomonas aeruginosa isolated from human and animal samples by multiplex-PCR and their antibiotic resistance pattern

Background: Pseudomonas aeruginosa is a leading cause of Hospital-acquired infection worldwide. A major problem in the treatment of bacterial infections is the emergence of strains with multiple resistances (MDR). The aim of this study was to identify virulence genes lasB, toxA, algD, exos in Pseudomonas aeruginosa isolates from human and animal by Multiplex-PCR method and determination of antibiotic resistance. Materials and methods: This cross-sectional study was performed on the 120 non-repetitive samples, including, 60 clinical samples of human and 60 animal samples collected from Tehran, Iran. Antibiotic susceptibility test was performed by disk diffusion test. The multiplex - PCR method was performed to identify virulence genes. Results: lasB and exoA genes were the most prevalent virulence determinants in the human and animals samples, respectively. The highest resistance rate was related to amoxicillin and amikacin in the both types of samples. Conclusion: Pseudomonas aeruginosa is an emerging threat to the production of antibiotic resistance genes and create strains of multidrug-resistant (MDR), serious problem growing that it is necessary to control emissions and to replace the antibiotics forever to reduce the prevalence of these genes, necessarily antibiotics new and appropriately.