Microsciadin, a New Milk-Clotting Cysteine Protease from an Endemic Species, Euphorbia microsciadia

نویسندگان

  • Hajar Rezanejad Molecular biotechnology laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz 71454, Iran
  • Reza Yousefi Protein chemistry laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran
  • Safoura Rezaei Molecular biotechnology laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz 71454, Iran
چکیده مقاله:

In the present work, a new branch of biotechnological advantage of the latex of an endemic perennial plant, Euphorbia microsciadia has been introduced. A novel cysteine protease, designated as microsciadin, was purified from the latex of Euphorbia microsciadia by a combination of sequential usage of SP-Sepharose Fast Flow column in two different pHs and a final gel filtration chromatography. Microsciadin is a monomeric protein with an apparent molecular mass of 60 kDa by SDS-PAGE. Although the enzyme was stable over a wide range of pH and temperatures, it displayed the maximum activity at 45 °C and pH of 4.5. The enzyme was strongly inhibited by Iodoacetamide, E-64 and Hg2+ ions indicated that it belongs to the cysteine protease family. Furthermore, the enzyme showed suitable stability in the presence of various denaturants and organic solvents. Moreover, primary studies on milk clotting activity of the enzyme revealed its high potential to dairy industry. The acidophilic feature of microsciadin in associated with its high milk-clotting activity and remarkable operational stability suggest its potential application in cheese industry, as well as other food and biotechnological fields.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

microsciadin, a new milk-clotting cysteine protease from an endemic species, euphorbia microsciadia

in the present work, a new branch of biotechnological advantage of the latex of an endemic perennial plant, euphorbia microsciadia has been introduced. a novel cysteine protease, designated as microsciadin, was purified from the latex of euphorbia microsciadia by a combination of sequential usage of sp-sepharose fast flow column in two different phs and a final gel filtration chromatography. mi...

متن کامل

Flavonol Glycosides from Euphorbia microsciadia Bioss. with their Immunomodulatory Activities

FOUR KNOWN FLAVONOIDS quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques....

متن کامل

Flavonol Glycosides from Euphorbia microsciadia Bioss. with their Immunomodulatory Activities

Four known flavonoids: quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques...

متن کامل

Flavonol Glycosides from Euphorbia microsciadia Bioss. with their Immunomodulatory Activities

Four known flavonoids: quercetin 3-O-β-D-rutinoside (Q3Rut), myricetin 3-O-β-D-galactopyranoside (M3Gal), quercetin 3-O-β-D-galactopyranoside (Q3Gal) and quercetin 3-O-β-D-glucopyranoside (Q3Glc), for the first time were isolated from aerial parts of Euphorbia microsciadia. The chemical structure of them was elucidated on the basis of 1 and 2 D-NMR spectra and different spectroscopic techniques...

متن کامل

Milk-clotting Enzymes from Microorganisms.

A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Prote...

متن کامل

مطالعه فیتوشیمیایی گیاه Euphorbia microsciadia

سابقه و هدف: خانواده فرفیون از جمله خانواده‌های بزرگ و مهم گیاهان دارویی هستند که دارای بیش از 800 گونه‌اند. این خانواده مهم از گیاهان را می‌توان در درمان بیماری‌های مختلفی نظیر سرطان و دردهای عصبی به کار برد. با توجه به اینکه در مورد گونه Euphorbia microsciadia تاکنون مطالعات فیتوشیمیایی انجام نگرفته است، در این تحقیق مورد بررسی قرار گرفت. مواد و روش‌ها: تحقیق به روش Exploratory survey انجام گ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 1

صفحات  93- 103

تاریخ انتشار 2015-07-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023