Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers

نویسندگان

  • Abbas Hassani Urmia University
  • Mahdieh Modareskia Urmia University
  • Maryam Kholghi Urmia University
  • Reza Darvishzadeh Urmia University
چکیده مقاله:

Study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. In the present study, genetic diversity among and within 15 Iranian native Ajowan(Carum copticum L.) populations were determined using inter simple sequence repeat (ISSR) markers. Twelve selected primers produced 153 discernible bands, with 93 (60.78%) being polymorphic, indicating considerable genetic diversity at the population level. Number of polymorphic bands per primer ranged from 4 to 11 with an average of 7.75. The largest percentage of polymorphic loci was 78.57% for primer UBC818. Based on an un-weighted pair-group method using arithmetic average (UPGMA) clustering algorithm, four distinct groups were established. Results of the principal coordinate analysis (PCoA) corresponded to those obtained through cluster analysis. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly within populations (61%), but variance among populations was only 39%. This study has detected significant genetic differentiation among C. copticum populations. The current study confirmed the importance of molecular studies in detecting genetic variation among genotypes in selecting diverse parents to carry out crossing program successfully.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

molecular diversity within and between ajowan (carum copticum l.) populations based on inter simple sequence repeat (issr) markers

study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. in the present study, genetic diversity among and within 15 iranian native ajowan(carum copticum l.) populations were determined using inter simple sequence repeat (issr) markers. twelve selected primers produced 153 discernible bands, with 93 (60.78%) being polymorph...

متن کامل

Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers.

Okra (Abelmoschus esculentus L.) is not only a nutrient-rich vegetable but also an important medicinal herb. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 24 okra genotypes. In this study, the PCR products were separated by electrophoresis on 8% nondenaturing polyacrylamide gel and visualized by silver staining. The 22 ISSR...

متن کامل

Maize (Zea mays L.) Genotypes Diversity Study by Utilization of Inter-Simple Sequence Repeat (ISSR) Markers

Analysis of the extent and distribution of genetic diversity in crop plants is essential for optimizing sampling and breeding strategies. Several methods were identified for the existence of diversity in maize for the aim of perfect selection based on great diversity among maize genotypes. In this study, Inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity in a sele...

متن کامل

Genetic Diversity of Marrubium Species from Zagros Region (Iran), Using Inter Simple Sequence Repeat Molecular Marker

This study concerns the genetic diversity and taxonomic status of Marrubium species from central and south-west of Zagros region, Iran. It is investigated by Inter-Simple Sequence Repeat analysis. A total of 68 accessions from five Marrubium species were collected from their natural habitats. Molecular analysis was approved with 17 primers, of which 12 were carried out in the reaction mixture. ...

متن کامل

Genetic diversity of Salix koreensis based on inter-simple sequence repeat (ISSR) in South Korea

Background Salix koreensisis native to Korea, Japan and China, is a deciduous broadleaved tall tree growing throughout Korea. This species is dioecius and planted along the streets and for landscaping. Along the rivers the species grows well reaching up to 20 meters in height. Trees bloom in April and fruits ripe in May. This study was carried out to investigate the genetic diversity of S. kore...

متن کامل

Molecular Characterization of Cultivated Bromeliad Accessions with Inter-Simple Sequence Repeat (ISSR) Markers

Bromeliads are of great economic importance in flower production; however little information is available with respect to genetic characterization of cultivated bromeliads thus far. In the present study, a selection of cultivated bromeliads was characterized via inter-simple sequence repeat (ISSR) markers with an emphasis on genetic diversity and population structure. Twelve ISSR primers produc...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 1

صفحات  51- 62

تاریخ انتشار 2012-12-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023