New p53 Gene Mutation in non-Cancerous Mustard Gas Exposed Lung
Objective Mustard gas (MG) is a poisoning chemical, mutagenic and carcinogenic alkylating agent. It is used during World War I and also Iran-Iraq conflict. The p53 tumor suppressor gene is involved in the pathogenesis of malignant disease. The aim of this study is to determine possible mutation in p53 gene of lung sample from mustard gas exposed patients. Material and Methods Twelve lung biopsy samples from 12 Mustard Gas exposed soldiers cases along with control cell line were studied for the presence of mutations in exons 4-9 of the p53 gene by PCR and direct sequencing. Results Among examined biopsies most of the samples demonstrated normal polymorphism with no significant defected mutations but in one sample one type of p53 gene alteration at codon 278 (CCT^CCA) on transcribed strand was detected. This Mutation has not been observed in another studies related to mustard gas exposure and p53 mutation databases. Conclusion In this study we have reported for the first time new p53 mutation in the lung sample of MG exposed patients. It is concluded that only one silent mutation were scanned with no signs of any type of cancer. This type of mutation was not in IARC p53 gene mutation database. Moreover, surrounding sequences of the mutated p53 gene codons have more 5'-GT and 5-GC sequences which have been found both by our study and only one another study on Japanese exposed to MG.
objective mustard gas (mg) is a poisoning chemical, mutagenic and carcinogenic alkylating agent. it is used during world war i and also iran-iraq conflict. the p53 tumor suppressor gene is involved in the pathogenesis of malignant disease. the aim of this study is to determine possible mutation in p53 gene of lung sample from mustard gas exposed patients. material and methods twelve lung biopsy...متن کامل
Pathologic Lesions of Liver, Kidney and Lung in the Autopsy of 100 Mustard Gas-Exposed Iranian War Veterans
Background & Objectives: Respiratory, central nervous system, and skin complications of mustard gas toxicity have previously been studied; however, the liver and kidney side effects due to this intoxication have not been fully noted. We aimed to evaluate the frequency of liver, kidney and lung lesions in mustard gas-exposed Iranian veterans who had been exposed to the toxin almost 2 decades bef...متن کامل
Background: Sulfur mustard (SM) is a chemical blistering warfare that affects different organs especially hematopoietic system. Prevalence of acute myeloblastic and lymphoblastic leukemia is increased by sulfur mustard exposure. FLT3-ITD mutation can be effective on leukemogenesis. Therefore, the aim of this study was to verify the frequency of FLT3-ITD mutation in the patients who exposed to S...متن کامل
CXCL10 Gene Expression in the Lung Tissue of Sulfur Mustard-Exposed Patients with Long-Term Pulmonary Complications
Aims: Mustard gas is an alkalizing substance that can lead to damage to DNA and intracellular enzymes. The aim of this study was to investigate the expression of CXCL10 in chemically-injured veterans’ lung tissue. Materials & Methods: This case-control study was done on 16 lung paraffin blocks of individuals exposed to mustard gas and 7 lungs paraffin blocks not-exposed people as...متن کامل
Objective(s):Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983–1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy and hypodiploidy. Studies have shown that the risk for acute myeloblastic and lymphoblastic leuke...متن کامل
EVALUATION OF SERUM IM MUNOGLOBULINS AND THEIR RELEVANCE TO CHRONIC LUNG DISEASE IN MUSTARD GAS VICTINIS
Despite the fact that 8 years has elapsed since the end of the imposed war, chemically injured victims are still suffering from various long-term complications, most of them respiratory in nature. The progressive nature of respiratory problems in these patients suggests that the immune system must be involved to Initiate a cascade which ends up causing lung injuries. To evaluate this, humo...متن کامل
دوره 10 شماره 2
صفحات 111- 117
تاریخ انتشار 2007-04-01
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