P-163: Effects of Bilateral Tubal Ligation on Serum Oxidative Stress and Ovarian Function in Female Rats

Background: Tubal ligation is one of the most common contraceptive methods in the world. Some researchers believe that tubal ligation has complications. It is assumed oxidative stress can produce these complications. The aim of this study was to evaluate oxidative stress and ovarian function after tubal ligation. Materials and Methods: In this study, female rats (weighting 200-250 g) were divided into 2 groups (tubal ligation and Sham). Serum (Prooxidant Antioxidant Balance), MDA (Malondialdehyde), estradiol and progesterone were measured on days 15 and 45 and 3 months and 6 months after the intervention in diestrous phase. Results: After tubal ligation, serum MDA increased however serum PAB increased after 15 days, 45 days, 3 months and then decreased after 6 months (p<0.05). Serum concentration of estradiol decreased remarkably,after 15 and 45 days (p<0.05). Serum concentration of progesterone decreased remarkably, after 15 days, 45 days, 3 months and 6 months in tubal ligation group compared to the sham group (p<0.05). Conclusion: Tubal ligation increased oxidative stress level and decreased estradiol and Progesterone of serum. Complications of tubal ligation may be due to increased oxidants and decreased estrogen and progesterone. As for the tubal ligation probably reduced serum estrogen levels, as a potent antioxidant, is involved in imbalance. By prescribing antioxidant, effects of tubal ligation can be reduced.