P-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate
Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but normal sperm chromatin strcture has been directly correlated with the rate of oocyte fertilization and embryo development. Our aim is to compare the effect of Swim up, DGC (with centrifugation) and Swim out (without centrifugation) techniques on spem DNA integrity by sperm chromatin dispersion (SCD) test and the fertilization rate. Materials and Methods: Semen sample was obtained from 207 patients referring to Montaserieh fertility and infertility center. 75 patients had leukospermia (>5×106 Not/ml) . Semen was analysed according to World Health Organization (WHO) criteria. Each semen sample was divided into 4 equal portions (Raw, Swim up, DGC, Swim out). Aliquots of each semen samples was measured for DNA fragmentation by SCD test. A total of 1863 oocytes were retrieved from patients and were injected with the husbands٫ prepared sperm. Then fertilization rate was evaluated on pronuclei stage. Results: For 207 patients, the level of DNA fragmentation in recovered sperms by 3 preparation methods was significantly lower than Raw semen (p<0.05). There was no significant difference in the percentage of DNA fragmentation and fertilization of recovered sperms by each of 3 methods(p>0.05). For 75 leukospermic patients, sperm DNA fragmentation after 3 methods was improved significantly compared with Raw semen. Sperm DNA fragmentation and fertilization rate significantly augmented in comparison with DNA integrity and Swimed up recovered sperms (p<0.05). Conclusion: Detrimental effect of sperm preparation techniques on sperm DNA fragmentation is related to the initial semen quality. It seems that use of Swim out method in leukospermic patients due to elimination of centrifugation step, which could stimulate ROS production by leukocytos can separate sperm with higher DNA integrity and increases fertilization rate.
P-37: Effect of Gibberellic Acid on Sperm DNA Double Strand Integrity, Nuclear Maturity and Motility
Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...متن کامل
Background: Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen parameters. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameters in these patients after...متن کامل
Effects of Sperm Acrosomal Integrity and Protamine Deficiency on In Vitro Fertilization and Pregnancy Rate
Background The objective of this study was to evaluate the relationship between protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in patients undergone in vitro fertilization (IVF). MaterialsAndMethods Semen samples from 70 infertile couples undergoing IVF at Isfahan Fertility and Infertility center were assessed in this study. Semen analysis was carried out ac...متن کامل
Effects of Sperm Chromatin Integrity on Fertilization Rate and Embryo Quality Following Intracytoplasmic Sperm Injection
Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opport...متن کامل
effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection
sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...متن کامل
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
دوره 7 شماره 3
صفحات 42- 42
تاریخ انتشار 2013-09-01
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