P-194: Investigation The Association of Leukemia Inhibitory Factor Gene Polymorphism with IVF Outcome in Infertile Women

Background: Clinical infertility is defined as the inability to become pregnant after 12 months of unprotected intercourse. Worldwide, more than 80 million couples suffer from infertility;the majority of this population are residents of developing countries. In vitro fertilization (IVF) is the most successful of the infertility treatments, and for many people is the last possibility for pregnancy. The implantation process is currently considered as the most relevant limiting factor for successful pregnancy. Thus, implantation failure of the trophoblast should be a major cause of repeated unsuccessful attempts at IVF. The embryo Leukemia inhibitory factor (LIF) is one of the key cytokines in the embryo implantation regulation and it is located at 22q12.2. The aim of this study was to investigate the C/T polymorphism in LIF gene for its involvement in IVF outcome. Materials and Methods: This study included 60 infertile women who underwent an IVF cycle and 57 healthy volunteers. Genomic DNA was extracted from peripheral blood leukocytes. Genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Statistical analysis was performed using the MedCalc program for Windows version 12. Results: No significant difference of the frequency of LIF gen polymorphism was observed between patients and control groups (p>0.05). Conclusion: LIF gene polymorphism may not be associated with IVF outcome in this population.