P-37: Effect of Gibberellic Acid on Sperm DNA Double Strand Integrity, Nuclear Maturity and Motility

Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s rats were used. The animals divided into two groups as; control and GA3 administrated (20 mg/ kg/day for 45 days, IP) animals. The acridine-orange staining was performed in order to investigate the sperm DNA damage and the aniline-blue staining was conducted to evaluate the percentage of sperms with nuclear maturity. The sperm motility was analyzed accordance to WHO standard method. Results: Florescent microscopic analyses showed that, the sperm DNA damage significantly (p<0.05) increased in GA3-dosed group. Moreover the GA3 administration remarkably (p<0.05) elevated the percentage of sperms with immature nuclei and reduced sperm motility as well. Meanwhile no pathological alterations occurred in control animal’s sperm content. Conclusion: Our results clarified that GA3 administration severely reduces sperm nuclear maturity and motility. Moreover GA3 consumption significantly increases sperm DNA damage.