P-64: Germinal Cells Intracytoplasmic Carbohydrate,Lipid and Lipase levels Alter in Longtime Varicocele-Induced Rats
نویسندگان
چکیده مقاله:
Background: Despite several studies many questions remain regarding how varicocele develops its adverse effect on testicular tissue. Spermatogenesis cell lineage needs carbohydrates as a main source of energy for cell division. Any disruption in glucose transporting system in seminiferous tubules results in remarkable decrease in germinal cells biological function. Therefore present study was conducted to evaluate the germinal cells intracytoplasmic carbohydrates, lipid foci and lipase enzyme levels in longtime varicocele-induced rats. Materials and Methods: To follow-up present study left varicocele was induced in test group (n=18). Non varicocelized rats were considered as control-sham. The test group further sub-divided into three groups based on the termination of the study and sample collecting date (4, 6 and 8 months after varicocele induction). The periodic acid schiff, Sudan-black B were performed in order to analyze intracytoplasmic carbohydrate and lipids accumulation, respectively. The lipase enzyme activity was evaluated by special lipase staining. Results: Observations demonstrated that in varicoceleinduced rats, the first three layers of germial cell lineage exhibited lower number of cells with periodic acid shift positive cytoplasm, higher number of cells with lipid positive stained cytoplasm in comparison to control animals.The lipase activity increased in 4 and 6 months varicoceles, while after 8 months the lipase activity significantly decreased. In contrast to test animals, the control-sham animals exhibited higher lipid accumulation in spermiogenesis cells lineage. Conclusion: Our data showed that after varicocele induction the germinal cells loss their major energy source (glucose) and switch the energy origins of carbohydrates to lipids. By decreased energy the lipase enzyme synthesis reduces, which in turn results in disruption of biological activity in germinal cells.
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عنوان ژورنال
دوره 6 شماره 2
صفحات -
تاریخ انتشار 2012-09-01
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