P-67: Quantitative Expression of Pluripotency Specific-Genes in Mouse Blastocysts Produced by In Vitro Fertilization
Background: The efficiency of in vitro fertilization (IVF) is still low to be developed to blastocyst stage probably because of environmental conditions. It is likely that in vitro environment can not exactly mimic in vivo environment due to differences in media, metabolic content, atmospheric composition, temperature and pH. Therefore it may affect embryo quality by changing in embryo gene expression. In this study, the expression level of pluripotency specific-genes: Oct4, Nanog and Sox2 were assessed in mouse blastocysts produced by IVF and compared with the control blastocysts flushed out of uterus on post coital day 3.5. Materials and Methods: Matured mouse oocytes were fertilized using capacitated sperms in HTF medium. Subsequently, presumptive zygotes were cultured for 4 days in KSOM medium up to the blastocyst stage. The expression levels of three mentioned genes were assessed by real-time PCR technique in IVF-derived blastocysts as treated group and in vivo collected blastocysts as control group. Results: Hatching rate of IVF-derived blastocysts was decreased significantly in comparison with the counterpart group (70.22% vs. 87.80%). Increase in Oct4, Nanog and Sox2 expression was observed in IVF versus in vivo generated mouse embryos. Conclusion: In vitro produced embryos followed by assisted hatching can be introduced as a reliable technique to help infertile couples.
Interfering Effects of In Vitro Fertilization and Vitrification on Expression of Gtl2 and Dlk1 in Mouse Blastocysts
Background: Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, thereis increasing concern that vitrification adversely affects embryo development. This study intends to assess the effectof vitrification on developmental competence, in addition to expressions of long non-coding RNA (lncRNA) gene traplocus 2 (Gtl2) and its reciprocal ...متن کامل
P-47: Effect of Blastocoelic Fluid Reduction on Quality and Expression of Developmentally Important Genes in Mouse Blastocysts
Background Recent researches reveal that manual puncturing of the trophectoderm of blastocyst before vitrification, increase the quality of embryo. However, in any of these studies, the importance of blastocoelic fluid and its impact on the formation of three cell lines is not mentioned. Therefore, in the present study, the effect of blastocoelic fluid reduction before vitrification on survival...متن کامل
Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...متن کامل
Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....متن کامل
BACKGROUND Human embryos produced by in vitro fertilization (IVF) are usually cultured to day 6 after insemination, and good quality of embryos should develop to blastocyst stage at days 5 and 6. However, some embryos develop slowly, thus they may form blastocysts on day 7. Most IVF laboratories culture embryos to day 6 and then discard retarded embryos that do not develop to blastocyst stage. ...متن کامل
P-23: Effect of Melatonin on In vitro Maturation, Fertilization and Resulted Embryo Development in Mouse
Background: Melatonin has been recognised as effective reactive oxygen species (ROS) scavenger. antioxident melatonin effects has been expressed in management of cancer, alzheimer, diabet diseases. This study designed to determine the effect of melatonin on in vitro oocytes maturation and fertilization and cleavage and blastocyst development rate of mouse. Materials and Methods: Cumulus - oocyt...متن کامل
دوره 8 شماره 2.5
صفحات 84- 84
تاریخ انتشار 2014-07-01
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