P-73: Morphological and Histochemical Detection of Proliferating Cells in Oovarian Follicle induced by Anticancer Drug Study

Background: Infertility problem affect more than 15% of young couples in different societies. One of the known causes of ovulation disorder is chemotherapy in patients with cancer. This side effect may last from ten years up to the end of the life. Since anticancer drugs are mainly insert their effect through decreasing of cell proliferation, the aim of the present study is to investigate, antiproliferative effect of anticancer drugs using electronmicroscopy and histochemical methods. Materials and Methods: A total of 30 bulb/c mice were used in this study. They were divided into control and thiotepa treated groups. Thiotepa was administrated as 2.5 mg /kg for 5 days. The mice were sacrificed after 5 weeks and testicular specimens were removed, fixed in boueins fixative and 2.5% Glutaraldehide then prepared for light and electronmicroscopic study. Results: light microscopy showed that the ovarian antral follicles was decreased in thiotepa group and many follicles have abnormal structure with decrease of cellular layers thickness, and electron microscopy revealed that in experimental group several vacuolated or ruptured mitochondria, nuclear chromatin was marginated and condensed with apoptotic cell features. Conclusion: It is concluded that thiotepa affect ovulation by both cytotoxic effect and decreasing of cell proliferation and differentiation.