P-87: Effects of Bovine Cumulus Cell Co-Cul tire on Post Fertilization Development of Buffalo (Bubalus bubalis) Oocyte
نویسندگان
چکیده مقاله:
Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo oocyte. Materials and Methods: Ovaries were collected from local slaughterhouse. The cumulus oocyte complexes (COC) were picked up form follicles (2-8 mm). The grade A and B COCs were randomly divided into 3 groups. Group 1: COCs were subjected to oocyte maturation medium containing TCM-199+ 5%FCS + 5%FF + 10mg/ml LH + 10 mg/ml FSH. Group 2: the COCs were cultured on a monolayer culture of bovine cumulus cells in a medium contained TCM199 with 5% FCS. Group 3: the denuded COCs were subjected on a monolayer culture of bovine cumulus cells in a medium similar to group 2. After 24 hours of maturation in an atmosphere with 5% CO2 and 95% relative humidity, oocytes subjected to in vitro fertilization using buffalo epididymal sperm. Three and six days after insemination (in BO medium) cleavage and blastocyst rates were evaluated. Results: Mean percentage of embryos cleaved for each were 41.3, 48.2 and 32.9 in groups 1, 2 and 3 (p>0.05), respectively. There was no developed embryo to blastocyst in all groups. Conclusion: The bovine cumulus co-culture with buffalo oocytes was effective as a completed in developmental potential of buffalo oocytes.
منابع مشابه
P-22: Effect of Bovine Cumulus Cell Co-Cultureon Maturation of Buffalo (Bubalus Bubalis)Oocyte
Background: The buffalo (Bubalus bubalis) is the animal with special production characteristics in producing milk and meat has a critical role in rural industry in Iran. The most problem of this specie is its low reproductive output. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on maturation rate of Buffalo oocyte.Materials and Methods: Ovaries were colle...
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عنوان ژورنال
دوره 6 شماره 2
صفحات -
تاریخ انتشار 2012-09-01
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