PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI

نویسندگان

  • MANA OLOOMI From the Molecular Biology Unit, Pasteur Institute of Iran, Tehran I.R. Iran.
  • SAEID BOUZARI
  • VLADIMIR O RECHINSKY
چکیده مقاله:

The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by immunoaffinity chromatography. The dot blot positive fractions were assayed for biological activity and it was shown that the expressed GM-CSF is active.

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عنوان ژورنال

دوره 12  شماره 4

صفحات  353- 357

تاریخ انتشار 1999-02

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