Simultaneous Detection of Integrase and Antibiotic Resistance Genes within SXT Constin in Vibrio cholerae O1 El Tor Strains Isolated from Iran Using Multiplex-PCR


  • Akbar Tavakoli Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
  • Mansoor Salehi Departments of Genetics, Isfahan University of Medical Sciences, Isfahan, Iran
  • Seyed Mahmoud Amin Marashi Department of Microbiology & Immunology, Babol University of Medical Sciences, Babol, Iran
  • Sharareh Moghim Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده مقاله:

Objective(s) Amongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. Materials and Methods Ninety four V. cholerae O1El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing dfrA1, sulII, strB and the int in a multiplex PCR were done. Results Results of our study showed that 92 (97.8%) out of 94 isolates were positive for all above genes. Multiplex PCR results correlated with the antibiotic susceptibility data obtained by using disc diffusion assay. Conclusion Using this multiplex PCR, it would be easily possible to demonstrate the presence of antibiotic resistance in SXT-C which, in turn, allows for a suitable treatment in cholera patients causing reduction in the mortality and morbidity rate of the infected individuals.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

simultaneous detection of integrase and antibiotic resistance genes within sxt constin in vibrio cholerae o1 el tor strains isolated from iran using multiplex-pcr

objective(s) amongst the various antibiotic resistant elements in vibrio. cholerae, sxt constin (sxt-c) is important. we were going to design a quick method for determination of antibiotic resistance gene pattern in sxt-c. materials and methods ninety four v. cholerae o1el tor isolates were used in this study. antibiotic susceptibility testing, multiplex pcr amplification of sxt-c containing df...

متن کامل

Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...

متن کامل

Variations in SXT elements in epidemic Vibrio cholerae O1 El Tor strains in China

Vibrio cholerae O1 El Tor biotype strains are responsible for three multiyear epidemics of cholera in China during the seventh ongoing pandemic. The presence of the integrative conjugative element SXT is strongly correlated with resistance to nalidixic acid, tetracycline, and trimethoprim-sulfamethoxazole in these strains. Here, we sequenced the conserved genes of the SXT element, including eex...

متن کامل

Vibrio cholerae O1 Hybrid El Tor Strains, Asia and Africa

To the Editor: Vibrio cholerae is a water-borne pathogen that causes a severe watery diarrhea disease known as cholera. On the basis of variable somatic O antigen composition, >200 serogroups of V. cholerae have been recognized. Classical and El Tor are 2 well-established biotypes within the V. cholerae O1 serogroup, and they can be distinguished by differences in their biochemical reactions or...

متن کامل

Molecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR

Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplific...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}

عنوان ژورنال

دوره 15  شماره 3

صفحات  885- 889

تاریخ انتشار 2012-05-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری

copyright © 2015-2023