Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle.

نویسندگان

  • A. Sotoodehnia
  • B. Omidi
  • G. Moazeni
  • S. Ataei
چکیده مقاله:

An oil adjuvant vaccine (OAV) was prepared from a local strain of Pasteurella multocida. Strain 6:B was cultured and inactivated by formalin. Bacterial pellet was prepared by centrifugation and subsequently adjuvanted by Montannide oil ISA-70. A dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by IM route. Animals were bled before and at 24, 90, 150, and 200 days post-vaccination. Collected sera were used in passive mouse protection test (PMPT). Active mouse protection test (AMPT) was carried out for OAV according to standard method. Results of PMPT showed 100% protection up to 150 days and 66-83% up to 200 days post-vaccination. In AMPT, 4 log of protection was gained. In this experiment the immunity induced by OAV adjuvanted by ISA-70 could protect the calves.

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منابع مشابه

study on immunity of an experimental oil adjuvant haemorrhagic septicaemia vaccine in cattle.

an oil adjuvant vaccine (oav) was prepared from a local strain of pasteurella multocida. strain 6:b was cultured and inactivated by formalin. bacterial pellet was prepared by centrifugation and subsequently adjuvanted by montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by im route. animals were bled before and at 24, 90, 150, and...

متن کامل

Haemorrhagic septicaemia in cattle and buffaloes*

Haemorrhagic septicaemia (HS) is a highly fatal disease in cattle and buffaloes, caused by specific serotypes of Pasteurella mul tocida. It is a primary pasteurellosis reproducible in susceptible animals with the specific organism alone. The accepted classification of Pasteurella multocida is based on the identification of its somatic and capsu­ lar antigens. On this basis two serotypes designa...

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عنوان ژورنال

دوره 59  شماره 2

صفحات  95- 101

تاریخ انتشار 2005-01-01

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