The Effect of Methylene Blue in Combination with Red Visible Light on Model Viruses Inactivation and Coagulation Factors in Fresh Frozen Plasma
Background and Aims: Fresh Frozen Plasma (FFP) is one of blood components. The risk of transmission of viruses from blood components regardless selection of blood donors and screening donated blood still remains. There are several methods for viral inactivation. In this study methylene blue (MB) photo inactivation process was used for inactivating viruses. Materials and Methods: In this study Methylene Blue(MB) was used in final concentration of 1µM. Infected Fresh Frozen Plasma (FFP) illuminated by 143Pieces (PCs) of 1 w red Light Emitting Diodes (LEDs) from two side for 10,15 and 30 minutes and shacked 30 cycle in minutes. The central wavelength of these LED is 627 nm with 20 nm Full Width at Half Maximum (FWHM).Herpes simplex virus-1(HSV-1) and vesicular stomatitis virus (VSV) were used as model viruses to evaluated illumination effects on viral inactivation. Level of fresh frozen plasma (FFP) coagulation factors such as fibrinogen, FV, FVIII, protein C, antitrombin measured pre and post illumination. Results: Initial HSV-1 and VSV titer were calculated to be 107 and 106.5 TCID50/ml, respectively .The level of viral inactivation was expressed as log-reduction. Titer reduction of HSV for 10, 15 and 30 minutes irradiation with shaking was>6, &ge7 and &ge 7 logs, respectively. The ratio of coagulation factors activity remaining unchanged after pathogen inactivation with MB calculated. Illumination had major effects on the mean levels of fibrinogen and FVIII. Significant differences between level of factors before and after illumination were evaluated with a t test for paired samples. No significant differences were seen in the FFP coagulation factors before and after illumination (P˃0.05). Conclusion: As results show the optimum time for viral inactivation were adjusted to be 15 minutes. Due to the reduction of virus titer at various times, agitation with illumination is effective.
inactivation of model viruses suspended in fresh frozen plasma using novel methylene blue based device.
background and objective: there is a concern on safety of human fresh frozen plasma (ffp) as it is a source of some medicinal products. the possibility of transmission of blood-borne are reported often due to emerging viruses. there are some pathogen reduction technologies (prt) to inactivate viruses. methylene blue (mb) based method is one of them. the aim of this study was to examine new desi...متن کامل
Inactivation of model viruses suspended in fresh frozen plasma using novel methylene blue based device
BACKGROUND AND OBJECTIVE There is a concern on safety of human Fresh Frozen Plasma (FFP) as it is a source of some medicinal products. The possibility of transmission of blood-borne are reported often due to emerging viruses. There are some Pathogen Reduction Technologies (PRT) to inactivate viruses. Methylene Blue (MB) based method is one of them. The aim of this study was to examine new desig...متن کامل
UNLABELLED Objectives: To determine the level of coagulation factors and inherited inhibitors in Fresh Frozen Plasma (FFP) and to evaluate Prothrombin Time and activated partial thrombin time in fresh frozen plasma. DESIGN Cross-sectional study. SETTING Jomo Kenyatta University of Agriculture and Technology in Medical Laboratory Sciences. SUBJECTS Eighteen blood bags collected from volunt...متن کامل
the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests
در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است15 صفحه اول
نتایج این مطالعه نشان می دهد که مقدار کلسیم (یونیزه و تام)، منیزیم و فسفر به طور معنی داری تغییر پیدا کرد به جزء مقدار کلسیم تام و منیزیم در روش داخل عضلانی که تغییر معنی داری نداشته است. بر اساس نتایج این مطالعه می توان نتیجه گیری کرد که اکسی تتراساکلین بر روی مقادیر سرمی کلسیم و منیزیم تأثیر می گذارد باید مقدار این کاتیون ها در بیماران، در هنگامی که اکسی تتراسایکلین استفاده می شود مورد توجه ق...15 صفحه اول
Hydrothermally synthesized nanocrystalline Nb2O5 and its visible-light photocatalytic activity for the degradation of congo red and methylene blue
Nb2O5 nanoparticles were synthesized by the hydrothermal method. Structural, morphological and elemental analysis of synthesized Nb2O5 nanoparticles was carried out using X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy, respectively. The average crystal size calculations were performed on the basis ...متن کامل
دوره 7 شماره None
صفحات 14- 20
تاریخ انتشار 2013-11
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