The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration

نویسندگان

  • Hassan Marashi Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Maziar Habibi Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Mohammadreza Nasiri Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Nasrin Moshtaghi Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Saeed Zibaee Razi Vaccine and Serum Research Institute, Mashhad, Iran.
  • Saeid Malekzadeh-Shafaroudi Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
چکیده مقاله:

Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-mediated transient gene expression assay was carried out in spinach (Spinacia oleracea) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of foreign gene and its expression in transformed plants were confirmed through polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. The results obtained in this examination showed quite a high level of gene expression in spinach leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Transient Expression of Foot and Mouth Disease Virus (FMDV) Coat Protein in Tobacco (Nicotiana tabacom) via Agroinfiltration

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

متن کامل

transient expression of coat protein of foot and mouth disease virus (fmdv) in alfalfa (medicago sativa) by agroinfiltration

an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...

متن کامل

transient expression of foot and mouth disease virus (fmdv) coat protein in tobacco (nicotiana tabacom) via agroinfiltration

background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants.  objective: the main goal of the present study was to eval...

متن کامل

Foot-and-mouth disease virus (FMDV) vaccines

Foot-and-mouth is a disease of global socioeconomic importance, with vaccination the principle tool for control in endemic countries. Improvements in vaccines require advances in our understanding of the host immune response, one aspect of which the study group is asked to consider. Major problems limit the capacity of FMDV vaccines to control infections in developing countries. The virus is no...

متن کامل

Expression of an epitope-based recombinant vaccine against Foot and Mouth Disease (FMDV) in tobacco plant (Nicotiana tabacum)

Regarding high potential of green plants for development of recombinant vaccines, this research was conducted to evaluate expression of a novel recombinant vaccines against Foot and Mouth Disease (FMDV) in tobacco plant. For this purpose, a synthetic gene encoding 129-169 amino acids of foot and mouth disease virus capsid protein VP1 was transferred to tobacco plant via Agrobacterium-mediated g...

متن کامل

Antigenic heterogeneity of capsid protein VP1 in foot-and-mouth disease virus (FMDV) serotype Asia 1

Foot and mouth disease virus (FMDV), with its seven serotypes, is a highly contagious virus infecting mainly cloven-hoofed animals. The serotype Asia1 occurs mainly in Asian regions. An in-silico approach was taken to reveal the antigenic heterogeneities within the capsid protein VP1 of Asia1. A total of 47 VP1 sequences of Asia1 isolates from different countries of South Asian regions were sel...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 2  شماره 2

صفحات  18- 27

تاریخ انتشار 2014-12-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023